Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody

Verified Samples in WB:COS-7
Verified Samples in IHC:Mouse kidney
Verified Samples in IF:Rat spleen

WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000

Polyclonal

IgG

Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/36

IκB-α (phospho Ser32/36)

I kappa B alpha;I-kappa-B-alpha;IkappaBalpha;IkB-alpha;IKBA;IKBA;IKBalpha;MAD 3;MAD3;Major histocompatibility complex enhancer-binding protein MAD3;NF kappa B inhibitor alpha;NF-kappa-B inhibitor alpha;NFKBI;NFKBIA;Nuclear factor of kappa light chain gene enhancer in B cells;Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha抗体

36kDa

34kDa

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Affinity purification

Cancer; Epigenetics and Nuclear Signaling; Immunology; Signal Transduction

Unconjugated

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.