本公司产品仅供体外研究使用，不用于临床诊断
本公司产品仅供体外研究使用，不用于临床诊断
中文名称:碘化丙啶（PI）染色液 (750 μM)
货号:E-CK-A165
4T1 cells and Hela cells were treated with 5 % DMSO, 5μM Camptothecin, 10μM Camptothecin for 4h, then stained with Calcein-AM / PI Double Staining Kit and photographed.
Molt-4 cells were treated with 5 % DMSO and 10 % DMSO for 4h, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.
Jurkat cells were placed at 4 °C for 20 days, then stained with Calcein-AM / PI Double Staining Kit and detected by flow cytometry.
PI是一种核酸染料，由于死细胞的细胞膜选择透过性丧失，碘化丙啶（Propidium Iodide，PI）可以进入胞内与双链DNA特异性结合并产生强烈的红色荧光 (Ex/Em=535nm/617nm)，从而对死细胞进行标记。
| 应用类型 | 细胞活力/细胞毒性 |
| 检测方法 | 荧光 |
| 样本类型 | 细胞 |
| 检测时间 | 30min |
| 检测仪器 | 流式细胞仪，荧光显微镜 |
| 荧光 | PI |
| Ex/Em (nm) | 535/617 |
| Channel set | PE/PerCP/Cy5.5 |
| Filter set | TRITC |
| 自备试剂 | PBS(E-BC-R187) |
| 保存温度 | -20°C, shading light |
| 运输条件 | 冰袋 |
| 保质期 | 12 months |
| E-CK-A153 | Calcein AM Assay Buffer |
| E-CK-A164 | Calcein AM Solution (100 μM) |
| E-CK-A354 | Calcein AM/PI Double Staining Kit |
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