Rat ANG2(Angiopoietin 2) ELISA Kit
中文名称：大鼠促血管生成素2(ANG2)酶联免疫吸附测定试剂盒
Price:
- 反应性： Rat
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠ANG2抗体包被于酶标板上，实验时样品（或标准品）中的大鼠ANG2会与包被抗体结合。后依次加入生物素化的抗大鼠ANG2抗体和辣根过氧化物酶标记的亲和素，抗大鼠ANG2抗体与结合在包被抗体上的大鼠ANG2结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，ANG2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中ANG2的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Rat |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的大鼠ANG2，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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IF: 7.100Journal： FREE RADICAL BIOLOGY AND MEDICINE (2024)Reactivity：RatSample Type：spinal cord tissue -
Chronic Intermittent Hypobaric Hypoxia Reduces Hypothalamic N-Methyl-d-Aspartate Receptor Activity and Sympathetic Outflow in Spontaneously Hypertensive Rats
IF: 2.100 -
N-Methyl-d-Aspartate-Induced Excitotoxicity and Its Impact on the Renin–Angiotensin System in Retinal Tissue
IF: 1.900Journal： JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS (2024)Reactivity：RatSample Type：retinal tissue -
Integrated serum pharmacochemistry and serum pharmacology to investigate the active components and mechanism of Bushen Huoxue Prescription in the treatment of diabetic retinopathy
IF: 3.400 -
Cold exposure, gut microbiota, and hypertension: A mechanistic study
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Candesartan Protects Against Cadmium-Induced Hepatorenal Syndrome by Affecting Nrf2, NF-κB, Bax/Bcl-2/Cyt-C, and Ang II/Ang 1–7 Signals
IF: 4.081 -
Effects of Ziyin Qianyang Formula on Renal Fibrosis through the TGF-β1/Smads Signaling Pathway in Spontaneously Hypertensive Rats
IF: 2.650Journal： Evidence-based Complementary and Alternative Medicine (2022)PMID：36387359Reactivity：RatSample Type：serum -
Qindan Capsule Attenuates Myocardial Hypertrophy and Fibrosis in Pressure Overload-Induced Mice Involving mTOR and TGF-β1/Smad Signaling Pathway Inhibition
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34007292Reactivity：MouseSample Type：Tissue homogenate -
Antihypertensive effects of Trichiurus lepturus myosin hydrolysate in spontaneously hypertensive rats
IF: 4.171 -
Synergistic effect of Aconiti Lateralis Radix Praeparata water-soluble alkaloids and Ginseng Radix et Rhizoma total ginsenosides compatibility on acute heart failure rats
IF: 2.813Journal： JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2019)PMID：31877430Reactivity：RatSample Type：Serum
实验操作视频
