Rat OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit
中文名称:大鼠氧化低密度脂蛋白(OxLDL)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.47-30 ng/mL
- 灵敏度: 0.28 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠OxLDL抗体包被于酶标板上,实验时样品(或标准品)中的大鼠OxLDL会与包被抗体结合。后依次加入生物素化的抗大鼠OxLDL抗体和辣根过氧化物酶标记的亲和素,抗大鼠OxLDL抗体与结合在包被抗体上的大鼠OxLDL结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,OxLDL浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中OxLDL的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.47-30 ng/mL |
灵敏度 | 0.28 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠OxLDL,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Ezetimibe treatment reduces oxidized low-density lipoprotein in biliary cirrhotic rats
IF: 3.000Journal: Journal of the Chinese Medical Association (2024)PMID:38380910Reactivity:RatSample Type:plasma -
Proprotein Convertase Subtilisin/Kexin Type 9 Inhibitor Improves the Vascular Function of Arteriovenous Fistula in Rats with Hyperglycemia
IF: 1.800Journal: Acta Cardiologica Sinica (2024)Reactivity:RatSample Type:serum -
PRDX6 Promotes Fatty Acid Oxidation via PLA2-Dependent PPARα Activation in Rats Fed High-Fat Diet
IF: 6.600 -
Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats
IF: 4.800 -
Edible Bird’s Nest Regulates Hepatic Cholesterol Metabolism through Transcriptional Regulation of Cholesterol Related Genes
IF: 2.650Journal: Evidence-based Complementary and Alternative Medicine (2022)PMID:35722163Reactivity:RatSample Type:serum -
A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition
IF: 5.863 -
The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet
IF: 5.719 -
Beneficial Effects of High Intensity Interval Training and/or Linseed Oil Supplementation to Limit Obesity-Induced Oxidative Stress in High Fat Diet-Fed Rats
IF: 5.719 -
Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model
IF: 4.868Journal: Oxidative Medicine and Cellular Longevity (2019)PMID:31396298Reactivity:RatSample Type:Plasma,Tissue homogenate -
Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity-related endothelial dysfunction in rat fed a high-fat diet
IF: 3.766Journal: PHYTOTHERAPY RESEARCH (2019)DOI:10.1002/ptr.6369PMID:31141276Reactivity:RatSample Type:blood