Rat TLR4(Toll-Like Receptor 4) ELISA Kit
中文名称:大鼠Toll样受体4(TLR4)酶联免疫吸附测定试剂盒

Price:
- 反应性: Rat
- 检测范围: 0.31-20 ng/mL
- 灵敏度: 0.19 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗大鼠TLR4抗体包被于酶标板上,实验时样品(或标准品)中的大鼠TLR4会与包被抗体结合。后依次加入生物素化的抗大鼠TLR4抗体和辣根过氧化物酶标记的亲和素,抗大鼠TLR4抗体与结合在包被抗体上的大鼠TLR4结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,TLR4浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TLR4的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Rat |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.31-20 ng/mL |
灵敏度 | 0.19 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的大鼠TLR4,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Activation of AMPK/mTOR-Driven Autophagy and Suppression of the HMGB1/TLR4 Pathway with Pentoxifylline Attenuates Doxorubicin-Induced Hepatic Injury in Rats
IF: 4.300 -
Testicular toxicity in cisplatin-treated Wistar rats is mitigated by Daflon and associated with modulation of Nrf2/HO-1 and TLR4/NF-kB signaling
IF: 3.600Journal: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2024)Reactivity:RatSample Type:Testicular tissue -
Neuroprotective effect of taxifolin against aluminum chloride-induced dementia and pathological alterations in the brain of rats: Possible involvement of toll-like receptor 4
IF: 3.200Journal: TOXICOLOGY MECHANISMS AND METHODS (2024)PMID:38465425Reactivity:RatSample Type:brain tissue -
Sirt1/Nrf2/TNFα; TLR4/Myd88/NF-κB signaling pathways are involved in mediating hepatoprotective effect of bupropion in rat model of myocardial infarction
IF: 2.900Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2024)Reactivity:RatSample Type:Hepatic tissue -
Macauba (Acrocomia aculeata) Pulp Oil Prevents Adipogenesis, Inflammation and Oxidative Stress in Mice Fed a High-Fat Diet
IF: 5.900 -
Averrhoa carambola L. fruit and stem metabolites profiling and immunostimulatory action mechanisms against cyclosporine induced toxic effects in rat model as analyzed using UHPLC/MS-MS-based chemometrics and bioassays
IF: 4.300 -
Ameliorative effects of vitamins-loaded flavoured nanophytosomes fortified with star anise volatile oil against CsA-Induced liver and kidney injury in rats: Application in functional ice cream.
IF: 4.000 -
Malva parviflora seed oil; Isolation, gas chromatographic profiling and its cardioprotective activity against myocardial infraction in animal model
IF: 3.800Journal: JOURNAL OF KING SAUD UNIVERSITY SCIENCE (2023)Reactivity:RatSample Type:plasma -
TLR4/ MyD88/NF-κB signaling pathway involved in the protective effect of diacerein against lung fibrosis in rats
IF: 2.800Journal: HUMAN & EXPERIMENTAL TOXICOLOGY (2023)PMID:37664986Reactivity:RatSample Type:Lung tissue -
Beta-glucan protects against isoproterenol-induced cardiac remodeling by regulating the ACE-AT1R axis and attenuates cardiac inflammation and apoptosis
IF: 1.700Journal: Asian Pacific Journal of Tropical Biomedicine (2023)Reactivity:RatSample Type:heart