Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)
中文名称：活性氧(ROS)荧光法测试盒(绿色)
Price:
- 样本类型： 组织;细胞
- 检测仪器及波长： 荧光酶标仪（Ex/Em=500 nm/525 nm），流式细胞仪
- 研究领域： 氧化应激,代谢疾病,铁死亡
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
DCFH-DA（2,7-dichlorofuorescin diacetate）是一种可以自由穿过细胞膜的荧光探针，自身没有荧光，进入细胞内后，可以被细胞内的酯酶水解生成DCFH（dichlorofluorescin）。在活性氧存在时DCFH被氧化为不能透过细胞膜的强绿色荧光物质DCF（dichlorofluorescein），其荧光在激发波长502nm，发射波长530nm附近有最大波峰，强度与细胞内活性氧水平成正比。
|
| 指标简称 | ROS |
| 检测时间 | 60 min |
| 检测仪器及波长 | 荧光酶标仪（Ex/Em=500 nm/525 nm），流式细胞仪 |
| 保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 6个月 |
-
Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms
IF: 14.903Journal：Acta Pharmaceutica Sinica B -
Comparative toxicity reduction potential of UV/sodium percarbonate and UV/hydrogen peroxide treatments for bisphenol A in water: An integrated analysis using chemical, computational, biological, and metabolomic approaches
IF: 9.13 -
Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice
IF: 8.101 -
Mangosteen Pericarp Extract Supplementation Boosts Antioxidant Status via Rebuilding Gut Microbiota to Attenuate Motor Deficit in 6-OHDA-Induced Parkinson’s Disease
IF: 7.675Journal：Antioxidants -
High-Fat-Diet–Induced Oxidative Stress Linked to the Increased Colonization of Lactobacillus sakei in an Obese Population | Microbiology Spectrum
IF: 7.171Journal：Microbiology Spectrum -
Parental cadmium exposure during the spawning period reduces cadmium sensitivity through the antioxidant system in rare minnow (Gobiocypris rarus) larvae
IF: 7.129 -
Alpinetin inhibits neuroinflammation and neuronal apoptosis via targeting the JAK2/STAT3 signaling pathway in spinal cord injury
IF: 7.035Journal：CNS Neuroscience & Therapeutics -
Potential Protective Mechanism in the Cardiac Microvascular Injury
IF: 6.823Journal：HYPERTENSION -
Docetaxel prodrug and hematoporphyrin co-assembled nanoparticles for anti-tumor combination of chemotherapy and photodynamic therapy
IF: 6.819Journal：DRUG DELIVERY -
The dispersion and utilization of lipid droplets mediates respiratory syncytial virus-induced airway hyperresponsiveness
IF: 6.377Journal：PEDIATRIC ALLERGY AND IMMUNOLOGY
Q1:Can the kit detect ROS in tissue homogenates?
This kit is suitable for detecting ROS in living cells. After tissue homogenate, cells have broken and died, so this kit cannot detect tissue homogenate.If it is a fresh tissue sample, a single cell suspension is prepared and can be detected.
Q2:What is the working concentration of reagent 1 and reagent 2? How many samples can the kit test?
There are differences among different samples. The specific concentration and action time of reagent 1 and the specific concentration and stimulation time of reagent 2 need to be explored by pre-experiment and determined by pre-experiment.The number of samples that can be detected by the kit depends on the concentration and dosage of reagent 1.
Q3:What fluorescence is detected by the ROS kit, and does the fluorescence of the sample itself affect the result?
It detects strong green fluorescence. If the sample carry fluorescent green there is interference, other color fluorescence has no effect on the kits.
