Recombinant SARS-CoV-2 Guanine-N7_meth Protein (His Tag)
Price:
Size:
50μg
10μg
- 表达系统: E.coli
- 蛋白编码: YP_009725309.1
别称 |
SARS-CoV 2 nsp14;SARS-CoV 2 ExoN;Guanine-N7 methyltransferase
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表达系统 |
E.coli
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序列 |
Ala1-Gln527
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蛋白编码 |
YP_009725309.1
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种属 |
SARS-CoV-2
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计算分子量 |
62.9 kDa
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表观分子量 |
60 kDa
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标签 |
N-His
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纯度 |
> 85 % as determined by reducing SDS-PAGE.
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内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
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保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
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制剂 |
Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 2mM DTT, 20% Glycerol, 0.1% TritonX-100, pH 6.0.
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复溶方法 |
Not Applicable
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背景 |
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.
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实验操作视频
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