• 本公司产品仅供体外研究使用,不用于临床诊断

Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)

  • Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)-Elabscience
<
>
  • Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Guanine-N7_meth Protein (His Tag)-Elabscience
    产品货号:PKSR030473

    别称:SARS-CoV 2 nsp14, SARS-CoV 2 ExoN, Guanine-N7 methyltransferase

    规格:
    • 10μg
    • 50μg
    数量:
    - +
    价格: ¥1760

    Expression_host: E.coli

    Accession: YP_009725309.1

    Overview

    Synonyms SARS-CoV 2 nsp14, SARS-CoV 2 ExoN, Guanine-N7 methyltransferase
    Expression host E.coli
    Sequence Ala1-Gln527
    Accesstion YP_009725309.1
    Species Virus
    Mol_Mass 62.9 kDa
    AP_Mol_Mass 60 kDa
    Tag N-6His

    Properties

    Form Lyophilized powder
    Purity > 85 % as determined by reducing SDS-PAGE.
    Endotoxin < 1.0 EU per g as determined by the LAL method.
    Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
    Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
    Formulation Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.
    Reconstitution Please refer to the printed manual for detailed information.
    Protein construction A DNA sequence encoding the Rat KLK6 (P36374-1) (Ile25-Pro261) was expressed with a polyhistidine tag at the N-terminus.
    Expression region Ile25-Pro261

    Background

    The nonstructural protein (nsp) 14 of 2019-nCoV was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). 2019-nCoV polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.

    写评论得优惠
    • 全部 (0)
    • 评论 (0)
    • 问答 (0)
    ... 展开 收起

    我的购物车

    产品名称 数量 价格
    结算

    总计:

    诚邀您参与Elabscience生化试剂盒性能验证计划还有积分奖励等你来赢!

    浏览历史


      买了该产品的人还买了