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Recombinant 2019-nCoV Helicase Protein (His Tag)

  • Recombinant Rat Alpha-fetoprotein Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Helicase Protein (His Tag)-Elabscience
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  • Recombinant Rat Alpha-fetoprotein Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Helicase Protein (His Tag)-Elabscience
  • Recombinant Rat Alpha-fetoprotein Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Helicase Protein (His Tag)-Elabscience
    产品货号:PKSR030466

    别称:SARS-CoV 2 Helicase, SARS-CoV 2 nsp13

    规格:
    • 10μg
    • 50μg
    数量:
    - +
    价格: ¥1760

    Expression_host: E.coli

    Accession: YP_009725308.1

    Overview

    Synonyms SARS-CoV 2 Helicase, SARS-CoV 2 nsp13
    Expression host E.coli
    Sequence Ala1-Gln601
    Accesstion YP_009725308.1
    Species Virus
    Mol_Mass 70 kDa
    AP_Mol_Mass 70 kDa
    Tag C-6His

    Properties

    Form Lyophilized powder
    Purity > 90 % as determined by reducing SDS-PAGE.
    Endotoxin < 1.0 EU per g as determined by the LAL method.
    Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
    Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
    Formulation Supplied as a 0.2 μM filtered solution of PBS, 10% Glycerol, pH 7.4.
    Reconstitution Please refer to the printed manual for detailed information.
    Protein construction A DNA sequence encoding the Rat Alpha-fetoprotein (P02773-1) (Arg25-Ala212) was expressed with a polyhistidine tag at the N-terminus.
    Expression region Arg25-Ala212

    Background

    The non-structural protein 13 (nsp13) of 2019-nCoV is a helicase that separates double-stranded RNA or DNA with a 5'-3' polarity, using the energy of nucleotide hydrolysis. A basic biochemical characterization of nsp13 demonstrated that it can unwind both doublestranded DNA and RNA in a 5’-3’ direction, and it can hydrolyze all deoxyribonucleotide and ribonucleotide triphosphates. Helicases are motor proteins that utilize the energy derived from nucleotide hydrolysisto unwind double-stranded nucleic acids into two single-stranded nucleic acids. Initially, helicases were only thought to be molecular engines that unwind nucleic acids during replication, recombination, and DNA repair. Recent studies have shown that they are also involved in other biological processes, including displacement of proteins from nucleic acid, movement of Holliday junctions, chromatin remodeling, catalysis of nucleic acid conformational changes, several aspects of RNA metabolism, including transcription, mRNA splicing, mRNA export, translation, RNA stability and mitochondrial gene expression. Some human diseases, including Bloom’s syndrome, Werner’s syndrome, and Xeroderma Pigmentosum have been associated with defects in helicase function.

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