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Recombinant 2019-nCoV NSP8 Protein (His Tag)

  • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience
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  • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV NSP8 Protein (His Tag)-Elabscience
    产品货号:PKSR030470

    别称:SARS-CoV 2 nsp8

    规格:
    • 10μg
    • 50μg
    数量:
    - +
    价格: ¥1760

    Expression_host: E.coli

    Accession: YP_009725304.1

    Overview

    Synonyms SARS-CoV 2 nsp8
    Expression host E.coli
    Sequence Ala1-Gln198
    Accesstion YP_009725304.1
    Species Virus
    Mol_Mass 25 kDa
    AP_Mol_Mass 25 kDa
    Tag C-6His

    Properties

    Form Lyophilized powder
    Purity > 95 % as determined by reducing SDS-PAGE.
    Endotoxin < 1.0 EU per g as determined by the LAL method.
    Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
    Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
    Formulation Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.5.
    Reconstitution Please refer to the printed manual for detailed information.
    Protein construction A DNA sequence encoding the Rat DNMT1 (Q9Z330-1) (Phe950-Pro1142) was expressed with a polyhistidine tag at the N-terminus.
    Expression region Phe950-Pro1142

    Background

    Cleavage by the viral main protease, 3CLpro results in generating the nsp8 protein, The nsp8 protein has been shown to associate with several other nsps and to colocalize with these nsps in cytoplasmic complexes that are important for viral RNA synthesis. It forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers. Nsp8 was shown to have RNA-dependent RNA polymerase (RdRp) activity that could be involved in producing primers utilized by nsp12 which is normally accepted to be the RdRp for SARS-CoV.

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