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Recombinant 2019-nCoV Papain-Like Protease Protein (Active)

  • Recombinant Rat KLK10 Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Papain-Like Protease Protein -Elabscience
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  • Recombinant Rat KLK10 Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Papain-Like Protease Protein -Elabscience
  • Recombinant Rat KLK10 Protein (His Tag)-Elabscience
  • Recombinant 2019-nCoV Papain-Like Protease Protein -Elabscience
    产品货号:PKSR030472

    别称:Papain-like Protease, PLpro, PL-PRO, pp1a, Replicase polyprotein 1a

    规格:
    • 10μg
    • 50μg
    数量:
    - +
    价格: ¥1760

    Expression_host: E.coli

    Accession: QHD43415.1

    Overview

    Synonyms Papain-like Protease, PLpro, PL-PRO, pp1a, Replicase polyprotein 1a
    Expression host E.coli
    Sequence Glu1564-Lys1878
    Accesstion QHD43415.1
    Species Virus
    Mol_Mass 35.8 kDa
    AP_Mol_Mass 34 kDa
    Bio_Activity Measured by its ability to cleave a fluorogenic peptide substrate, (E-EDANS)RELNGGAPI(K-DABCYL)S (Synthetic).The specific activity is >2000pmoi/min/mg.

    Properties

    Form Lyophilized powder
    Purity > 90 % as determined by reducing SDS-PAGE.
    Endotoxin < 1.0 EU per g as determined by the LAL method.
    Storage Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
    Shipping This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
    Formulation Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, 10 mM Mercaptoethanol [BME], 20% Glycerol, pH 7.5.
    Reconstitution Please refer to the printed manual for detailed information.
    Protein construction A DNA sequence encoding the Rat KLK10 (P36375-1) (Pro19-Pro259) was expressed with a polyhistidine tag at the N-terminus.
    Expression region Pro19-Pro259

    Background

    Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.

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