请输入货号/产品关键词 !
插入特殊字符:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • 收起 ↑
热门搜索: 最新活动 文献引用 新品速递 一步法ELISA 流式抗体 铜死亡 Annexin V凋亡检测 TUNEL凋亡检测 未包被ELISA 铁死亡 双硫死亡

Recombinant SARS-CoV-2 Spike Protein (RBD, mFc Tag)

Price: ¥2024 ¥22080

Size:
50μg 1mg
  • 表达系统: HEK293 Cells
  • 蛋白编码: QHD43416.1
  • 应用性: ELISA
别称
S protein RBD;2019-nCoV S protein RBD
表达系统
HEK293 Cells
序列
Asn331-Val524
蛋白编码
QHD43416.1
种属
SARS-CoV-2
计算分子量
47.9 kDa
表观分子量
50-60 kDa
标签
C-mFc
生物活性
Immobilized Human ACE-2-His(Cat#PKSH032068) at 10μg/ml (100 μl/well) can bind Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030474). The ED50 of Recombinant 2019-nCoV Spike Protein (RBD, mFc Tag) (Cat#PKSR030474) is 62 ng/ml.
纯度
> 95 % as determined by reducing SDS-PAGE.
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method.
保存条件
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
运输条件
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
制剂
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
复溶方法
Not Applicable
背景
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.


微信客服 微信客服 在线留言 TOP