Recombinant Human Galectin-3 Protein(Gst Tag)

货号：PDEH100582
说明书

说明书

Recombinant Human Galectin-3 Protein(Gst Tag)

Price: ¥4500.00 ¥1500.00

Size:

100μg 20μg

表达系统： E.coli
蛋白编码： P17931

别称	MAC;Galactose-specific lectin;Galactoside-Binding Soluble;Galectin;Lectin L;GAL;CBP;LGALS3;CBP35;GAL3;GALBP;GALIG;L31;LGALS2;MAC2;Galactoside-Binding Soluble 3;Lectin;Galectin-3;Gal-3;L-31;35 kDa lectin;CBP 35;Galactose-specific lectin 3;IgE-binding protein;Laminin-binding protein;Lectin L-29;Mac-2 antigen;Carbohydrate binding protein 35;Carbohydrate-binding protein 35;galactose binding;galactoside binding soluble 3;Galactoside-binding protein;galectin 3;Galectin 3 internal gene;Galectin3;GBP;IgE binding protein;included;L 31;L 34;L-34 galactoside-binding lectin;Lectin;Galactoside-Binding Soluble 3;LEG3;MAC 2 antigen;Mac-2;Macrophage galactose-specific lectin;MGC105387;P35;soluble 3
表达系统	E.coli
序列	Ala2-Ile250
蛋白编码	P17931
种属	Human
计算分子量	53.4 kDa
表观分子量	53 kDa
标签	N-Gst
生物活性	Not validated for activity
纯度	> 90 % as determined by reducing SDS-PAGE.
内毒素	< 10 EU per mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

SDS-PAGE analysis of Human Galectin-3 proteins, 2μg/lane of Recombinant Human Galectin-3 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 53 KD

Q1:无标签蛋白纯化方式？

蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。

Q2:蛋白Buffer里是否含有denaturants（变性剂）？

我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q5:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。

Q6:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。