Recombinant Human NRP2 Protein(His Tag)

货号：PDMH100178
说明书

说明书

Price: ¥4500.00 ¥1500.00

Size:

100μg 20μg

表达系统： Mammalian
蛋白编码： O60462

别称	MGC126574;Neuropilin 2;Neuropilin-2;Neuropilin2;NP 2;NP2;NPN 2;NPN2;NRP 2;Nrp2;NRP2;PRO2714;Receptor for VEGF165 and semaphorins class3;Vascular endothelial cell growth factor 165 receptor 2;Vascular endothelial growth factor 165 receptor 2;VEGF1265R2;VEGF165R2
表达系统	Mammalian
序列	Val20-Glu855
蛋白编码	O60462
种属	Human
计算分子量	91.9 kDa
表观分子量	90-95 kDa
标签	C-His
生物活性	Not validated for activity
纯度	> 90 % as determined by reducing SDS-PAGE.
内毒素	< 1.0 EU per mg of the protein as determined by the LAL method
保存条件	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件	This product is provided as lyophilized powder which is shipped with ice packs.
制剂	Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
复溶方法	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
背景	This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with an vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

SDS-PAGE analysis of Human NRP2 proteins, 2 μg/lane of Recombinant Human NRP2 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 91.9 KD

Q1:无标签蛋白纯化方式？

蛋白的纯化方式一般通过标签进行亲和纯化，无标签的蛋白是在纯化后进行了标签切除。

Q2:蛋白Buffer里是否含有denaturants（变性剂）？

我们在售重组蛋白一般不含变性剂，具体见说明书的formulation信息。常见变性剂有：尿素和盐酸胍。

Q3:蛋白的运输条件是什么？

我们的蛋白有液体和冻干粉2种形式，一般来说冻干粉可以用冰袋、蓝冰、干冰运输；液体可以用蓝冰或干冰运输。

Q4:重组蛋白有活性吗？可以做活性检测吗？

活性说明的见说明书bio-activity部分；如果说明书显示没有生物活性或则显示生物活性检测中，则说明这个蛋白没有生物活性数据，需要客户自己检测。

Q5:蛋白发货形式可以是冻干粉吗？可以发液体吗？

默认按照说明书标注的形式发货；如果有特殊需求，需要根据蛋白情况确定。原则上，冻干粉蛋白可以发液体形式，液体蛋白不能发冻干粉形式。

Q6:蛋白是通过什么方式纯化的？

一般通过纯化标签进行亲和纯化，常规有镍柱/His Tag、protein AG/Fc tag、标签抗体凝胶等。