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Recombinant Human TNF-alpha/TNFA Protein

Price: ¥1184 ¥592

Size:
50μg 10μg
  • 表达系统: E.coli
  • 蛋白编码: P01375
  • 应用性: Cell culture
别称
Tumor Necrosis Factor;Cachectin;TNF-Alpha;Tumor Necrosis Factor Ligand Superfamily Member 2;TNF-a;TNF;TNFA;TNFSF2
表达系统
E.coli
序列
Val77-Leu233
蛋白编码
P01375
种属
Human
计算分子量
17.5 kDa
表观分子量
16 kDa
标签
None
生物活性
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml.
纯度
> 95 % as determined by reducing SDS-PAGE.
内毒素
< 0.01 EU per μg of the protein as determined by the LAL method.
保存条件
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
运输条件
This product is provided as lyophilized powder which is shipped with ice packs.
制剂
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
复溶方法
Please refer to the printed manual for detailed information.
背景
TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation; differentiation; inflammation; viral replication; tumorigenesis; autoimmune diseases and in viral; bacterial; fungal; and parasitic infections. Besides inducing hemorrhagic necrosis of tumors; TNF was found to be involved in tumorigenesis; tumor metastasis; viral replication; septic shock; fever; inflammation; and autoimmune diseases including Crohn’s disease; and rheumatoid arthritis as well as graft-versus-host disease.


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