Recombinant SARS-CoV-2 (SARS-CoV-2) S protein RBD (C-mFc-6His tag)
Price:
Size:
100μg
50μg
- 表达系统: HEK293 Cells
- 蛋白编码: QHD43416.1
- 应用性: ELISA
别称 |
S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19
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表达系统 |
HEK293 Cells
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序列 |
Arg319-Phe541
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蛋白编码 |
QHD43416.1
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种属 |
SARS-CoV-2
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计算分子量 |
52.2 kDa
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标签 |
C-mFc-His
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生物活性 |
1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng/ml.
2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein(PKSR030492) in a linear range of 0.488-49.83 ng/ml.
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纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
内毒素 |
Please contact us for more information.
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保存条件 |
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
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运输条件 |
This product is provided as lyophilized powder which is shipped with ice packs.
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制剂 |
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
复溶方法 |
Please refer to the printed manual for detailed information.
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背景 |
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
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1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody(E-AB-V1028) in a linear range of 0.24-15.62 ng/ml.
2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein(PKSR030492) in a linear range of 0.488-49.83 ng/ml.
实验操作视频
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