Super Excellent Chemiluminescent Substrate (ECL)Detection Kit
中文名称:超敏化学发光 (ECL)底物液
Price:
Size:
500mL
100mL
50mL
- 应用性: WB;ELISA
产品简介 | Elabscience®超敏化学发光 (ECL) 底物液是一款辣根过氧化物酶 (HRP) 底物,可用于检测辣根过氧化物酶(HRP)标记的蛋白或核酸,用于蛋白质印迹或ELISA等分析蛋白含量,可兼容胶片和数字显影系统。 本产品所含特有成分能有效降低非特异发光和背景发光,并减缓信号衰减,使得30 min后信号值无明显减弱,120 min后发光强度仍能达到最高发光强度的约70%左右。 |
保存条件 | 2~8°C避光保存,保质期12个月。 |
注意事项 | 1. 曝光背景高,可能是膜没洗干净、二抗或一抗浓度过高、封闭液不合适导致,请做适当调整。 2. A液和B液在吸取过程中必须要更换吸头,A液和B液相互污染后会导致其逐渐失效。 3. 如无信号,可能是目的蛋白表达弱,可适当延长曝光时间。 4. 荧光迅速淬灭,可能是目的条带荧光信号过强,导致HRP快速消耗ECL底物,建议降低二抗浓度。 5. NaN3或金属螯合剂会抑制HRP 活性,溶液中应避免使用。 6. 工作液需现配现用。 |
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E-IR-R307 | Excellent Chemiluminescent Substrate (ECL)Detection Kit | 500mL , 100mL , 50mL |