CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method)
中文名称:分型超氧化物歧化酶(CuZn-SOD/Mn-SOD)测试盒(羟胺法)
Price:
Size:
96T(92 samples)
48T(44 samples)
- 样本类型: 血清(浆);尿液;动植物组织;细胞;细胞上清
- 检测仪器及波长: 酶标仪(530-570 nm,最适检测波长550 nm)
- 研究领域: 氧化应激
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基(O2.-),它氧化羟胺形成亚硝酸盐,在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后,催化超氧阴离子自由基发生歧化反应,使形成的亚硝酸盐减少,进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值,来计算SOD的活性。同时,经过试剂处理的样本导致Mn-SOD失活,而不影响CuZn-SOD。
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指标简称 | CuZn-SOD;T-SOD |
灵敏度 | 1.35 U/mL |
检测范围 | 1.35-62 U/mL |
精密度 | 批间差:9.6 | 批内差:5.1 |
检测时间 | 70 min |
检测仪器及波长 | 酶标仪(530-570 nm,最适检测波长550 nm) |
保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存6个月,具体试剂组分的保存条件请参考说明书。 |
有效期 | 6个月 |
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E-BC-K019-M | Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method) | 96T(92 samples) , 48T(44 samples) |
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