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T(Testosterone) ELISA Kit

  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
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  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
  • competitive-ELISA-Elabscience
    产品货号:E-EL-0155c

    中文名:睾酮( Testosterone)酶联免疫吸附测定试剂盒

    别称:Testosterone,T

    规格:
    • 96T
    • 48T
    数量:
    - +
    价格: ¥2000

    反应性: 通用

    检测范围: 0.31~20 ng/mL

    灵敏度: 0.17 ng/mL

    货期: 3个工作日+顺丰周期

    加入购物车 比较 咨询 说明书

    用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中天然T浓度。

    检测原理

    本试剂盒采用竞争ELISA法。用T抗原包被于酶标板上,实验时样品或标准品中的T与包被的T竞争生物素标记的抗T单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,T浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中T的浓度。

    反应类型 竞争ELISA
    规格 96T
    反应时间 2.0h
    反应性 通用
    检测方法 比色法
    检测范围 0.31-20 ng/mL
    灵敏度 0.17 ng/mL
    样本体积 50μL
    样本类型 Serum, plasma, saliva and urine

    特异性

    可检测样本中的T,且与其它相关蛋白无明显交叉反应。

    重复性

    板内、板间变异系数均<10%。

    典型数据

    由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    (ng/mL) O.D Average
    20 0.275
    0.263
    0.269
    10 0.523
    0.567
    0.545
    5 0.782
    0.698
    0.74
    2.5 1.027
    0.985
    1.006
    1.25 1.238
    1.36
    1.299
    0.62 1.618
    1.598
    1.608
    0.31 1.837
    2.001
    1.919
    0 2.41
    2.442
    2.426

    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
    板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

      Intra-assay Precision Inter-assay Precision
    Sample 1 2 3 1 2 3
    n 20 20 20 20 20 20
    Mean
    (ng/mL)
    1.33 5.22 10.88 1.54 5.73 10.02
    Standard
    deviation
    0.11 0.35 0.46 0.14 0.35 0.68
    C V (%) 8.13 6.72 4.27 9.23 6.18 6.75

    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。

    Sample Type Range (%) Average Recovery (%)
    Serum (n=5) 80-90 84
    EDTA plasma (n=5) 82-94 87
    Urine (n=5) 85-102 95

    线性

    分别往5个样本中添加已知浓度的目标物,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。

        Serum (n=5) EDTA plasma (n=5) Urine (n=5)
    1:2 Range (%) 83-90 88-108 85-101
    Average (%) 85 96 93
    1:4 Range (%) 80-95 87-95 80-102
    Average (%) 88 89 92
    1:8 Range (%) 89-100 82-89 83-96
    Average (%) 93 86 88
    1:16 Range (%) 87-115 87-105 84-99
    Average (%) 97 98 87

    试剂盒组成及保存

    未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。

    中文名称 英文名称 规格 保存条件
    ELISA酶标板(可拆卸) Micro ELISA Plate(Dismountable) 8 孔×12 条 -20℃,可存放6个月
    冻干标准品 Reference Standard 2支
    浓缩生物素化抗体(100×) Concentrated Biotinylated Detection Ab 1支 120 μL
    浓缩HRP酶结合物(100×) Concentrated HRP Conjugate 1支 120 μL -20℃(避光),可存放6个月
    标准品&样品稀释液 Reference Standard & Sample Diluent 1瓶 20 mL 2-8℃.可存放6个月
    生物素化抗体稀释液 Biotinylated Detection Ab Diluent 1瓶 14 mL
    酶结合物稀释液 HRP Conjugate Diluent 1瓶 14 mL
    浓缩洗涤液(25×) Concentrated Wash Buffer (25×) 1瓶 30 mL
    底物溶液(TMB) Substrate Reagent 1瓶 10 mL 2-8℃(避光)
    反应终止液 Stop Solution 1瓶 10 mL 2-8℃
    封板覆膜 Plate Sealer 5 张
    产品说明书 Product Description 1 份
    质检报告 Certificate of Analysis 1 份

    说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
    试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。

    试验所需自备物品

    • 1.酶标仪(450nm波长滤光片)
    • 2.高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
    • 3.37℃恒温箱,双蒸水或去离子水
    • 4.吸水纸

    操作步骤

    1. 对应板孔中加入50μL标准品工作液或样本后,立即每孔加入50μL生物素化抗体工作液,37℃孵育45分钟

    2. 弃掉板内液体,洗板3次

    3. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次

    4. 每孔加入90μL底物溶液,37℃孵育15分钟左右

    5. 每孔加入50μL终止液

    6. 立即在450nm波长下读数,处理数据

    客户发表文献

    1. Expression of FKBP52 in the ovaries of PCOS rats
      IF: 2.784
      Journal: International Journal of Molecular Medicine(2018)
      DOI: 10.3892/ijmm.2018.3998
      PMID: 30483787
      引用产品: E-EL-R0391c E-EL-R0026c E-EL-0150c E-EL-0154c E-EL-0155c 
      样本类型: Serum
      物种: Rat
    2. Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes.
      IF: 6.023
      Journal: Diabetologia(2018)
      DOI: 10.1007/s00125-018-4755-3
      PMID: 30411254
      引用产品: E-EL-0155c 
      样本类型: Cell culture supernatant
      物种: Human
    3. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats.
      IF: 2.776
      Journal: Environmental toxicology(2018)
      DOI: 10.1002/tox.22559
      PMID: 29663635
      引用产品: E-EL-0155c 
      样本类型: Tissue homogenate
      物种: Rat
    4. Short Communication Combined Effects of Vitamin C and Tomato Extract (Lycopersicon Esculentum) on Carbimazole-induced Alterations in the Testes of Male Albino Rats.
      IF: /
      Journal: Indian J Physiol Pharmacol(2018)
      引用产品: E-EL-0155c 
      样本类型: Serum
      物种: Rat
    5. CTRP3 attenuates high-fat diet-induced male reproductive dysfunction in mice.
      IF: 4.936
      Journal: Clinical Science(2018)
      DOI: 10.1042/CS20180179
      PMID: 29572383
      引用产品: E-EL-0155c 
      物种: Mouse
    6. Adequate Exercise Improves Inflammatory, Gonadal Markers and Sexual Function in Type 2 Diabetics.
      IF: /
      Journal: Journal of Advances in Medicine and Medical Research(2018)
      DOI: 10.9734/JAMMR/2018/39766
      引用产品: E-EL-0155c 
      物种: Human
    7. Dual Effects Of Sulfasalazine On Rat Sperm Characteristics, Spermatogenesis, And Steroidogenesis In Two Experimental Models.
      IF: 3.858
      Journal: Toxicology Letters(2017)
      DOI: 10.1016/j.toxlet.2017.11.034
      PMID: 29197623
      引用产品: E-EL-0155c 
      样本类型: Serum
      物种: Rat
    8. Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells.
      IF: 2.806
      Journal: Plos One(2017)
      DOI: 10.1371/journal.pone.0188029
      PMID: 29125859
      引用产品: E-EL-0150c E-EL-0155c E-EL-0154c 
      样本类型: Cell culture supernatant
      物种: Universal
    9. ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats.
      IF: 5.965
      Journal: Cell Death & Disease(2017)
      DOI: 10.1038/cddis.2017.494
      PMID: 29072679
      引用产品: E-EL-0154c E-EL-0155c E-EL-0150c E-EL-H1143c E-EL-H0532c E-EL-R0026c E-EL-R0391c E-EL-R0017c 
      样本类型: Serum
      物种: Universal
    10. Diet-Induced Obesity Impairs Spermatogenesis: A Potential Role For Autophagy.
      IF: 4.259
      Journal: Scientific Reports(2017)
      DOI: 10.1038/srep43475
      PMID: 28276438
      引用产品: E-EL-0150c E-EL-0155c E-EL-M0511c E-EL-M2747c 
      样本类型: Serum
      物种: Universal, Mouse
    11. Protective Effects Of Fluvastatin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet Through Enhanced Signaling Of mTOR.
      IF: 5.104
      Journal: Cellular Physiology and Biochemistry(2017)
      DOI: 10.1159/000457881
      PMID: 28214901
      引用产品: E-EL-R0026c E-EL-R0391c E-EL-0155c E-EL-0150c 
      样本类型: Serum
      物种: Rat
    12. Disruption Of Sex-Hormone Levels And Steroidogenic-Related Gene Expression On Mongolia Racerunner (Eremias Argus) After Exposure To Triadimefon And Its Enantiomers.
      IF: 4.208
      Journal: Chemosphere(2017)
      DOI: 10.1016/j.chemosphere.2016.12.096
      PMID: 28039834
      引用产品: E-EL-0155c E-EL-0150c 
      样本类型: Plasma
      物种: Horse
    13. InVitro And InVivo Evaluation Of Tissue-Cultured Mountain Ginseng OnPenile Erection.
      IF: 4.082
      Journal: Journal of Ginseng Research(2016)
      DOI: 10.1016/j.jgr.2015.10.003
      PMID: 27746685
      引用产品: E-EL-0155c 
      样本类型: Serum
      物种: Rat
    14. Curcumin Ameliorates HighFat DietInduced Spermatogenesis Dysfunction.
      IF: 1.692
      Journal: Molecular Medicine Reports(2016)
      DOI: 10.3892/mmr.2016.5712
      PMID: 27600729
      引用产品: E-EL-0150c E-EL-0155c E-EL-R0391c E-EL-R0026c E-EL-R0582c 
      样本类型: Serum
      物种: Rat
    15. Curcumin Ameliorates High-Fat Diet-Induced Spermatogenesis Dysfunction.
      IF: 1.692
      Journal: Molecular Medicine Reports(2016)
      DOI: 10.3892/mmr.2016.5712
      PMID: 27600729
      引用产品: E-EL-0150c E-EL-0155c E-EL-R0391c E-EL-R0026c E-EL-R0582c 
      样本类型: Serum
      物种: Rat
    16. Brain Thromboxane A2 Via Arachidonic Acid Cascade Induces The Hypothalamic-Pituitary-Gonadal Axis Activation In Rats.
      IF: 2.225
      Journal: Autonomic Neuroscience-Basic & Clinical(2015)
      DOI: 10.1016/j.autneu.2015.02.005
      PMID: 25784152
      引用产品: E-EL-R0391c E-EL-0071c E-EL-R0026c E-EL-0155c 
      样本类型: Plasma
      物种: Rat
    17. Protective Effects Of Metformin On Reproductive Function In Obese Male Rats Induced By High-Fat Diet.
      IF: 2.163
      Journal: Journal of Assisted Reproduction & Genetics(2015)
      DOI: 10.1007/s10815-015-0506-2
      PMID: 26081124
      引用产品: E-EL-0150c E-EL-0155c E-EL-R0391c E-EL-R0026c E-EL-R0582c 
      样本类型: Serum
      物种: Rat
    18. Differential Proteome And Gene Expression Reveal Response To Carbon Ion Irradiation In Pubertal Mice Testes.
      IF: 3.858
      Journal: Toxicology Letters(2014)
      DOI: 10.1016/j.toxlet.2014.01.001
      PMID: 24440814
      引用产品: E-EL-M0511c E-EL-0155c 
      样本类型: Serum
      物种: Mouse
    19. Analysis of gene expression and functional changes of adrenal gland in a rat model of kidney yang deficiency syndrome treated with Sini decoction.
      IF: 1.41
      Journal: Experimental and Therapeutic Medicine
      DOI: 10.3892/etm.2018.6521
      PMID: 30214533
      引用产品: E-EL-0155c 
      样本类型: Serum
      物种: Rat
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