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TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
  • sandwich-Ab-ELISA-Elabscience
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    产品货号:E-EL-0162c

    中文名:转化生长因子β1(TGF-β1)酶联免疫吸附测定试剂盒

    别称:Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1

    规格:
    • 96T
    • 48T
    • 96T*5
    数量:
    - +
    价格: ¥2000

    反应性: Universal

    检测范围: 0.16~10 ng/mL

    灵敏度: 0.10 ng/mL

    货期: 3个工作日+顺丰周期

    在线咨询 比较 加入购物车 说明书

    用途

    该试剂盒用于体外定量检测血清、血浆或其他相关生物液体中TGF-β1浓度。


    背景介绍

    转化生长因子β1 (TGF-β1)是一种具有强力抗炎特性的多效性细胞因子,由于其参与脂质代谢、高血压和动脉粥样硬化的病理生理进程,被认为是缺血性卒中(IS)炎症反应过程中重要的高危因素。TGF-β1的基因位于19号染色体 (q13.1-13.3),包括7个外显子和6个内含子。TGF-β1蛋白遍布全身,在构成骨架的组织中尤为丰富,在早期发育过程中,骨架由强韧而富有弹性的骨骼和软骨组织构成,TGF-β1可以帮助调节这些组织的形成与发育。TGF-β1还参与了血管的形成,肌肉和脂肪组织的发育,伤口的愈合,免疫系统中的炎症反应进程并能预防肿瘤的生长。


    检测原理

    本试剂盒采用双抗体夹心ELISA法。用抗TGF-β1抗体包被于酶标板上,实验时样品或标准品中的TGF-β1会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗GF-β1抗体和辣根过氧化物酶标记的亲和素。抗TGF-β1抗体与结合在包被抗体上的TGF-β1结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,TGF-β1浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TGF-β1的浓度。

    反应类型

    夹心法

    规格

    96T

    反应时间

    3.5h

    反应性

    通用

    检测方法

    Colormetric

    检测范围

    0.1610 ng/mL

    灵敏度

    0.10 ng/mL

    样本体积

    100μL

    样本类型

    血清、血浆或其他相关生物液体

    特异性

    可检测样本中的TGF-β1,且与其它相关蛋白无明显交叉反应

    重复性

    板内,板间变异系数均<10%


    样品活化方法

    生物样品中的通常以无活性的形式存在,在检测TGF-β1活性前必须进行活化处理。

    活化方法如下:

    血清、血浆: 280 μL标准品&样品稀释液中加入40 μL样品,混匀,加入40 μL活化试剂1,室温孵育10分钟。再加入40 μL活化试剂2,混匀后立即检测。

    注意:样品被稀释了10倍!

    细胞培养上清:20 μL标准品&样品稀释液中加入100 μL样品,混匀,加入40 μL活化试剂1,室温孵育10分钟。再加入40 μL活化试剂2,混匀后立即检测。

    注意:样品被稀释了2倍!


    样本值

    血清/血浆:本实验对来自健康的人、大小鼠等样本进行了TGF-β1水平检测。

    样本类型

    样本来源

    样本值范围

    稀释倍数

    血清

    健康人(n=10)

    22.8-46.1 ng/mL

    10

    血浆

    健康人(n=10)

    12.7-43.1 ng/mL

    10

    血清

    健康小鼠(n=10)

    66.1-82.9 ng/mL

    10

    血清

    健康大鼠(n=10)

    35.4-66.5 ng/mL

    10

    血清

    健康猪(n=5)

    4.9-10.2 ng/mL

    10

    血清

    健康兔(n=5)

    2.9-6.5 ng/mL

    10

    血清

    健康犬(n=5)

    34.5-84.2 ng/mL

    10


    典型数据

    以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。

    ng/mL

    10

    5

    2.5

    1.25

    0.63

    0.32

    0.16

    0

    OD

    2.515

    1.645

    0.988

    0.506

    0.271

    0.183

    0.111

    0.057

    校正OD

    2.458

    1.588

    0.931

    0.449

    0.214

    0.126

    0.054

    -








    精密度

    板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

     

    批内变异系数

    批间变异系数

    样本

    1

    2

    3

    1

    2

    3

    数量

    20

    20

    20

    20

    20

    20

    平均值( MERGEFIELD 单位ng/mL)

    0.41

    1.07

    4.96

    0.47

    1.25

    4.24

    标准差

    0.02

    0.05

    0.22

    0.03

    0.06

    0.2

    变异系数 (%)

    4.88

    4.67

    4.43

    6.38

    4.8

    4.72


    回收率

    分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。


    样本类型


    回收率范围 (%)

    平均回收率 (%)


    血清(n=5)


    94-110

     102


    血浆(EDTA)(n=5)


    90-102

     96


    细胞上清(n=5)


    95-106

     98















    客户发表文献

    1. Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy
      IF: 27.398
      Journal: Advanced materials(2019)
      DOI: 10.1002/adma.201905751
      PMID: 31709671
      样本类型: Tissue homogenate
      物种: Mouse
    2. Virus-like nanoparticle as a co-delivery system to enhance efficacy of CRISPR/Cas9-based cancer immunotherapy
      IF: 10.317
      Journal: Biomaterials(2020)
      DOI: 10.1016/j.biomaterials.2020.120275
      样本类型: Serum,Cell lysate,Tissue homogenate
      物种: Mouse
    3. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility
      IF: 6.638
      Journal: Acta Biomaterialia(2019)
      DOI: 10.1016/j.actbio.2019.05.012
      PMID: 31075515
      样本类型: Cell culture supernatant
      物种: Human
    4. Suppression For Lung Metastasis By Depletion Of Collagen I And Lysyl Oxidase Via Losartan Assisted With Paclitaxel-Loaded Ph-Sensitive Liposomes In Breast Cancer.
      IF: 6.402
      Journal: Drug Delivery(2016)
      DOI: 10.3109/10717544.2015.1132798
      PMID: 26758229
      样本类型: Cell lysate
      物种: Human
    5. Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome
      IF: 5.717
      Journal: Nutrients(2021)
      DOI: 10.3390/nu13051602
      PMID: 34064762
      样本类型: Tissue homogenate
      物种: Mouse
    6. Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model
      IF: 5.116
      Journal: Stem Cell Research & Therapy(2020)
      DOI: 10.1186/s13287-020-01733-4
      PMID: 32456711
      样本类型: Plasma
      物种: Mouse
    7. Aspirin Reduces Cardiac Interstitial Fibrosis by Inhibiting Erk1/2-Serpine2 and P-Akt Signalling Pathways.
      IF: 5.104
      Journal: Cellular Physiology and Biochemistry(2018)
      DOI: 10.1159/000487972
      PMID: 29518782
      物种: Mouse
    8. Long Non-Coding RNA-ATB Promotes EMT During Silica-Induced Pulmonary Fibrosis By Competitively Binding miR-200c.
      IF: 5.476
      Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2017)
      DOI: 10.1016/j.bbadis.2017.11.003
      PMID: 29113749
      样本类型: Cell culture supernatant
      物种: Human
    9. Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites
      IF: 4.471
      Journal: International Journal of Nanomedicine(2019)
      DOI: 10.2147/IJN.S184920
      PMID: 30666115
      样本类型: Cell culture supernatant
      物种: Rat
    10. Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.
      IF: 4.401
      Journal: J Cell Sci(2018)
      DOI: 10.1242/jcs.216135
      PMID: 29898921
      样本类型: Tissue homogenate
      物种: Rat
    11. Effect Of Qianghuo Erhuang Decoction On T Regulatory And T Helper 17 Cells In Treatment Of Adjuvant-Induced Arthritis In Rats.
      IF: 4.259
      Journal: Scientific reports(2017)
      DOI: 10.1038/s41598-017-17566-w
      PMID: 29222448
      样本类型: Serum
      物种: Rat
    12. DHCR24 overexpression modulates microglia polarization and inflammatory response via Akt/GSK3β signaling in Aβ 25-35 treated BV-2 cells
      IF: 3.647
      Journal: Life Sciences(2020)
      DOI: 10.1016/j.lfs.2020.118470
      PMID: 32950573
      样本类型: Cell sample
      物种: Mouse
    13. Effects of catalpol on asthma by airway remodeling via inhibiting TGF-β1 and EGF in ovalbumin-induced asthmatic mice
      IF: 3.375
      Journal: American Journal of Translational Research(2020)
      DOI: 99999999
      PMID: 32774762
      样本类型: Serum
      物种: Mouse
    14. I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis
      IF: 3.758
      Journal: Mediators of Inflammation(2020)
      DOI: 10.1155/2020/6947482
      PMID: 32256194
      样本类型: Serum,Cell culture supernatant,Aqueous Humor
      物种: Mouse
    15. Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway
      IF: 3.943
      Journal: International Immunopharmacology(2020)
      DOI: 10.1016/j.intimp.2020.106287
      PMID: 32058932
      样本类型: Tissue homogenate
      物种: Mouse
    16. Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression
      IF: 3.647
      Journal: Life sciences(2019)
      DOI: 10.1016/j.lfs.2019.116659
      PMID: 31323274
      样本类型: Serum
      物种: Human
    17. The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP
      IF: 3.212
      Journal: Inflammation(2019)
      DOI: 10.1007/s10753-019-01030-0
      PMID: 31209730
      样本类型: Serum
      物种: Mouse
    18. Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2019)
      DOI: 10.1016/j.biopha.2019.108706
      PMID: 30844656
      样本类型: Wound drainage fluid
      物种: Human
    19. Pterostilbene alleviates fructose-induced renal fibrosis by suppressing TGF- β1/TGF-β type I receptor/Smads signaling in proximal tubular epithelial cells
      IF: 3.04
      Journal: European Journal of Pharmacology(2018)
      DOI: 10.1016/j.ejphar.2018.10.008
      PMID: 30336139
      样本类型: Serum,Cell culture supernatant
      物种: Human
    20. The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice.
      IF: 3.273
      Journal: Toxins(2018)
      DOI: 10.3390/toxins10110449
      PMID: 30384460
      样本类型: Serum, Tissue homogenate
      物种: Mouse
    21. Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS.
      IF: 3.098
      Journal: Molecules(2017)
      DOI: 10.3390/molecules22111956
      PMID: 29137154
      样本类型: Cell culture supernatant
      物种: Rat
    22. Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.
      IF: 3.457
      Journal: Biomedicine & Pharmacotherapy(2018)
      DOI: 10.1016/j.biopha.2018.07.081
      PMID: 30119204
      样本类型: Serum
      物种: Rat
    23. Cordyceps Cicadae NTTU 868 Mycelium Prevents CCl 4-Induced Hepatic Fibrosis In BALB/c Mice Via Inhibiting The Expression Of Pro-Inflammatory And Pro-Fibrotic Cytokines.
      IF: 3.144
      Journal: Journal of Functional Foods(2018)
      DOI: 10.1016/j.jff.2018.02.010
      物种: Mouse
    24. Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.
      IF: 3.226
      Journal: International Journal of Molecular Sciences(2015)
      DOI: 10.3390/ijms160922062
      PMID: 26378531
      样本类型: Serum,Tissue homogenate
      物种: Mouse
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      IF: 2.907
      Journal: Future Microbiology(2020)
      DOI: 10.2217/fmb-2020-0005
      PMID: 32250184
      样本类型: Serum
      物种: Rat
    26. A Peptide from Kiwifruit Exerts Anti-Inflammatory Effects in Celiac Disease Mucosa
      IF: 2.175
      Journal: Journal of the American College of Nutrition(2019)
      DOI: 10.1080/07315724.2018.1541426
      PMID: 30794064
      样本类型: Tissue homogenate
      物种: Human
    27. Two Sulfur Glycoside Compounds Isolated From Lepidium Apetalum Willd Protect NRK52e Cells Against Hypertonic-Induced Adhesion And Inflammation By Suppressing The MAPK Signaling Pathway And RAAS.
      IF: 2.861
      Journal: Molecules(2017)
      DOI: 10.3390/molecules22111956
      PMID: 29137154
      样本类型: Cell culture supernatant
      物种: Rat, Universal
    28. Edaravone Attenuates Lipopolysaccharide-induced Acute Respiratory Distress Syndrome Associated Early Pulmonary Fibrosis Via Inhibition Of Oxidative Stress And Transforming Growth Factor-β1/Smad3 Signaling.
      IF: 2.466
      Journal: Biochemical And Biophysical Research Communications(2017)
      DOI: 10.1016/j.bbrc.2017.10.165
      PMID: 29102631
      样本类型: Serum
      物种: Rat
    29. Concentrated Growth Factor Promotes Schwann Cell Migration Partly Through The Integrin Β1-Mediated Activation Of The Focal Adhesion Kinase Pathway.
      IF: 2.341
      Journal: International Journal of Molecular Medicine(2016)
      DOI: 10.3892/ijmm.2016.2520
      PMID: 26986804
      样本类型: Cell culture supernatant
      物种: Rat
    30. Galectin-9 Ameliorates Respiratory Syncytial Virus-Induced Pulmonary Immunopathology Through Regulating The Balance Between Th17 And Regulatory T Cells.
      IF: 2.628
      Journal: Virus Research(2014)
      DOI: 10.1016/j.virusres.2014.10.011
      PMID: 25451068
      样本类型: Tissue homogenate
      物种: Rat
    31. Effects And Mechanisms Of Geniposide On Rats With Adjuvant Arthritis.
      IF: 2.956
      Journal: International Immunopharmacology(2014)
      DOI: 10.1016/j.intimp.2014.02.021
      PMID: 24583144
      样本类型: Cell lysate
      物种: Rat
    32. Histone deacetylase inhibitor suberoylanilide hydroxamic acid alleviates liver fibrosis by suppressing the transforming growth factor-β1 signal pathway
      IF: 1.5
      Journal: Hepatobiliary & Pancreatic Diseases International(2018)
      DOI: 10.1016/j.hbpd.2018.09.013
      PMID: 30249543
      样本类型: Serum
      物种: Rat
    33. SMOC1 silencing suppresses the angiotensin IIinduced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway.
      IF: 1.664
      Journal: Oncology Letters(2018)
      DOI: 10.3892/ol.2018.8989
      PMID: 30127878
      样本类型: Plasmid
      物种: Mouse
    34. Leukocyte Depletion Does Not Affect The In Vitro Healing Ability Of Platelet Rich Plasma.
      IF: 1.785
      Journal: Experimental and Therapeutic Medicine(2018)
      DOI: 10.3892/etm.2018.5887
      PMID: 29556269
      物种: Human
    35. Foxp3+ Associated With The Pro-Inflammatory Regulatory T And T Helper 17 Effector Cells In Asthma Patients.
      IF: 1.261
      Journal: Experimental and Therapeutic Medicine(2016)
      DOI: 10.3892/etm.2016.3662
      PMID: 27703517
      样本类型: Plasma
      物种: Human
    36. Pirfenidone Suppresses MAPK Signaling Pathway To Reverse Epithelial-Mesenchymal Transition And Renal Fibrosis.
      IF: 1.563
      Journal: Nephrology(2016)
      DOI: 10.1111/nep.12831
      PMID: 27245114
      样本类型: Tissue homogenate
      物种: Rat
    37. Anti-Oxidant And Anti-Inflammatory Effects Of Apigenin In A Rat Model Of Sepsis: An Immunological, Biochemical, And Histopathological Study.
      IF: 1.475
      Journal: Immunopharmacology & Immunotoxicology(2016)
      DOI: 10.3109/08923973.2016.1173058
      PMID: 27144896
      样本类型: Serum
      物种: Rat
    38. Comparison of Transforming Growth Factor-Beta 1 Concentration in Preeclampsia and Normal Pregnancy Women
      Journal: The Indonesian Biomedical Journal(2017)
      DOI: 10.18585/inabj.v9i1.310
      样本类型: Serum
      物种: Human
    39. Possible cause-and-effect linkage of transforming growth factor-beta1 and platelets derived growth factor-AB with delayed anthropometric parameters in adolescent patients with Cooley's anemia: Cases vis control research strategy
      Journal: Eurasian Journal of Biosciences(2020)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    40. Relationship of levels of transforming growth factor-beta1 (TGF-β1) to the levels of ferritin in blood of transfusion dependent β-thalassemia major patients with growth retardation: A case-control study
      Journal: Eurasian Journal of Biosciences(2020)
      DOI: 99999999
      样本类型: Serum
      物种: Human
    41. Mechanistic Insight Into the Efficient Osteogenic Potential of Dihydrotestosterone: Exploring Sequential Expression of Bone-Related Protein Biomarkers.
      Journal: Acta Medica Iranica(2018)
      样本类型: Cells
      物种: Mouse
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    Q

    S****rSubmitted [ Feb 25 2023 ]

    问: TGF β标准品需要活化吗 为什么样品复孔间差异特别大

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