Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)
中文名称：总超氧化物歧化酶(T-SOD)比色法测试盒(羟胺法)
Price:
Size:
96T(92 samples)
48T(44 samples)
- 样本类型： 血清（浆）;尿液;动植物组织;细胞;细胞上清
- 检测仪器及波长： 酶标仪（540-560 nm，最佳550 nm）
- 研究领域： 氧化应激,植物生理系列
该指标在测定组织或细胞样本时，需要测定样本的总蛋白含量，推荐使用BCA法测蛋白，货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通，可进行售前验证
根据需测样本数，试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量，请点击按钮 计算
生化试剂盒数量计算器x
需测样本数：
单个样本测量孔数：
试剂盒测样本孔数：
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量：
| 检测原理 |
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基（O2.-），它氧化羟胺形成亚硝酸盐，在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后，催化超氧阴离子自由基发生歧化反应，使形成的亚硝酸盐减少，进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值，来计算SOD的活性。
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| 指标简称 | T-SOD |
| 灵敏度 | 2.4 U/mL |
| 检测范围 | 2.4-61 U/mL |
| 精密度 | 批间差：5.6 | 批内差：5.5 |
| 检测时间 | 70 min |
| 检测仪器及波长 | 酶标仪（540-560 nm，最佳550 nm） |
| 保存条件 | 该产品可在 2~8°C/-20°C避光条件下保存6个月，具体试剂组分的保存条件请参考说明书。 |
| 有效期 | 6个月 |
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| E-BC-K020-M | Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) | 96T(92 samples) , 48T(44 samples) |
| E-BC-K022-M | CuZn/Mn Superoxide Dismutase (CuZn-SOD/Mn-SOD) Activity Assay Kit (Hydroxylamine Method) | 96T(92 samples) , 48T(44 samples) |
Q1:For SOD detection, can the blood sample be anticoagulant with EDTA?
The activity center of SOD is metal ions, and the use of EDTA as an anticoagulant will chelate metal ions, which may cause the decrease of SOD enzyme activity and affect the results.
Q2:To detect SOD in cell samples, is it feasible to treat cells with RIPA lysis buffer?
SDS in RIPA lysis buffer interferes with enzyme activity determination, so it is not recommended to use RIPA lysis buffer for cell treatment. The SOD kit requires that the sample should not contain SDS, Tween20, NP-40, Triton X-100 and other detergents, and should not contain DTT, 2-mercaptoethanol and other reducing reagents.
