Triton X-100
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- 应用性: IF
产品简介 | Triton X-100(聚乙二醇辛基苯基醚) 是一种非离子型表面活性剂(或称去污剂)。分子量为646.86(C34H62O11)。它能溶解脂质,以增加细胞膜对抗体的通透性。
免疫细胞化学中Triton X-100常用浓度为1%和0.3%,其中1%的Triton X-100常用于漂洗组织标本,0.3%的Triton X-100则常用于稀释血清,0.1%的Triton X-100常用于LAMP(环介导等温扩增),配制BSA等。 通常是先配制成30%的Triton X-100储备液,临用时稀释至所需浓度。 |
保存条件 | 室温保存,有效期12个月。 |
注意事项 | 1. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。 2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 |
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Q1:细胞裂解一般需要多大浓度的Triton X-100呢?一般使用什么溶液进行稀释?
使用Triton X-100裂解细胞时浓度一般为1%;稀释时可使用PBS或者TBS。