Vimentin Polyclonal Antibody

Verified Samples in WB:Hela,HepG2,SW480,B16,PC12,Mouse lung
Verified Samples in IHC:Human lung cancer,Human colon,Human liver cancer,Human kidney

WB 1:500-1:2000, IHC 1:200-1:800

Polyclonal

IgG

KLH conjugated Synthetic peptide corresponding to Mouse vimentin

Vimentin

CTRCT30;Epididymis luminal protein 113;FLJ36605;HEL113;VIM;VIME;Vimentin抗体

57kDa

57kDa

Cytoplasm

1.1 mg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Affinity purification

Cancer; Developmental Biology; Neuroscience; Signal Transduction; Stem Cells

Unconjugated

Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.