(KO Validated) Vimentin Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC;IF
Verified Samples |
Verified Samples in WB:Jurkat,293T,HeLa,Mouse lung,Mouse testis,Rat lung Verified Samples in IHC:Human tonsil,Human stomach Verified Samples in IF:L929 |
Dilution | WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant fusion protein of human Vimentin (NP_003371.2). |
Abbre | Vimentin |
Synonyms | CTRCT30;HEL113;Vimentin;VIM;vimentin抗体 |
Swissprot | |
Calculated MW | 53kDa |
Observed MW | 57kDa |
Cellular Localization | Cytoplasm |
Concentration | 1mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Purification Method | Affinity purification |
Research Areas | Cancer; Developmental biology; Neuroscience; Signaling transduction; Stem cells |
Conjugation | Unconjugated |
Background | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. |
Western blot analysis of extracts of various cell lines using Vimentin Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells using Vimentin Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunohistochemistry of paraffin-embedded Human stomach using Vimentin Polyclonal Antibody at dilution of 1:150 (40x lens).
Immunofluorescence analysis of L929 cells using Vimentin Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |