Western Blot Detection Kit
中文名称:Western Blot试剂盒
Price:
- 应用性: WB
产品简介 | WB,Western Blot(免疫印迹实验)是生物科学领域检测蛋白质表达最常用和最经典的实验方法。然而由于不同实验人员所用试剂来源、操作方法和经验的区别,导致大量WB实验结果不符合文章发表要求。Elabscience专注于免疫学领域多年,积累了大量的WB操作经验。为帮助科研人员解决上述问题,现特推出Western Blot检测试剂盒。试剂盒内为经典WB实验所需试剂,包含了从蛋白提取到ECL底物曝光在内的所有试剂,具有操作简便、检测灵敏度高、背景低、系统稳定性强等优点,并提供详尽的操作说明和专业的技术支持,为您顺利做出理想的Western Blot实验结果提供了便捷和保障。
由于PVDF膜有两种孔径,分别适用于转不同分子量大小的目的蛋白;为了满足不同实验需求,我们设计了两种规格的Western Blot试剂盒: E-IR-R304A(PVDF膜0.45 μm孔径)建议用于检测分子量大于20 kDa的蛋白质; E-IR-R304B(PVDF膜0.22 μm孔径)建议用于检测分子量小于20 kDa的蛋白质。 |
保存条件 | 室温/ 2~8°C/-20°C,详见说明书,有效期12个月 |
注意事项 | 1. 本试剂盒仅用于科研,不能应用于临床。 2. 收到该试剂盒后,请按照说明书所提供的保存条件进行保存。 3. 请注意安全事项,遵守实验室试剂操作规范操作。 4. 为了您的安全和健康,请穿实验服并戴一次性手套操作。 |
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E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500 μL , 120 μL , 60 μL , 30 μL , 1 mL |
E-BC-K318-M | BCA Protein Colorimetric Assay Kit | 500 Assays , 96 T (80 samples) |
E-BC-R187 | PBS Buffer, pH7.4 (10×) | 500 mL , 200 mL , 100 mL |
E-BC-R266 | PVDF Membrane (0.45 μm) | 8.5 cm × 6 cm × 10 pieces , 8.5 cm × 6 cm × 20 pieces , 13.25 cm × 15 cm × 10 pieces , 13.25 cm × 15 cm × 20 pieces |
E-BC-R273 | Pre-stained Protein Marker (10~180 kDa) | 500 μL , 250 μL |
E-BC-R288 | 5× SDS Loading Buffer | 10 mL , 5 mL , 2 mL |
E-BC-R327 | RIPA Lysis Buffer (Strong) | 100 mL , 50 mL , 20 mL |
E-BC-R337 | Skim Milk Powder | 50 g , 10 g , 5 g |
E-IR-R305 | SDS-PAGE Gel kit | 50 Assays , 25 Assays , 10 Assays |
E-IR-R307 | Excellent Chemiluminescent Substrate (ECL)Detection Kit | 500 mL , 100 mL , 50 mL |
Q1:请问一下用贵公司的WB试剂盒的话,需要哪些仪器设备?
做WB实验需要的仪器,超声仪,水浴锅,移液枪及枪头,离心机,电泳槽,SDS-PAGE电泳仪,转膜仪,摇床,wb成像系统仪器。
Q2:E-IR-R304A Western Blot Detection Kit的优势?
本试剂盒为经典WB实验提供全套试剂,包括从蛋白质提取到结果检测所需试剂,具有操作简单、检测灵敏度高、背景低、系统稳定性强等优点。
Q3:货号E-IR-R304A和E-IR-R304B的WB实验试剂盒里面是否包含E-IR-R305凝胶试剂?
E-IR-R305凝胶试剂盒是不包含在E-IR-R304A和E-IR-R304B里面的,需要单独购买凝胶试剂盒;
Q4:E-IR-R304A/E-IR-R304B区别?
由于PVDF膜有两种孔径,分别适用于转不同分子量大小的目的蛋白, 为了满足不同实验需求,我们设计了两种规格 E-IR-R304A(PVDF膜0.45 μm孔径)建议用于检测分子量大于20 kDa的蛋白质 E-IR-R304B(PVDF膜0.22 μm孔径)建议用于检测分子量小于20 kDa的蛋白质。
Q5:试剂盒内有没有WB实验所需的海绵垫和滤纸?
E-IR-R304A和E-IR-R304B试剂盒有包含滤纸,但不含海绵垫;