ACH(Acetylcholine) ELISA Kit
中文名称:乙酰胆碱(ACH)酶联免疫吸附测定试剂盒
别称:
ACh
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用ACH抗原包被于酶标板上,实验时样品或标准品中的ACH与包被的ACH竞争生物素标记的抗ACH单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,ACH浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中ACH的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 50μL |
样本类型 | 血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的ACH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
The effect of the calcium channel blocker nimodipine on hippocampal BDNF/Ach levels in rats with experimental cognitive impairment
IF: 2.529Journal:NEUROLOGICAL RESEARCH -
Differentiation of human glioblastoma U87 cells into cholinergic neuron
IF: 2.173 -
Effects of Euterpe oleracea to Enhance Learning and Memory in a Conditioned Nicotinic and Muscarinic Receptor Response Paradigm by Modulation of Cholinergic Mechanisms in Rats
IF: 2.02 -
The analysis of hippocampus neuronal density (CA1 and CA3) after Ocimum sanctum ethanolic extract treatment on the young adulthood and middle age rat model
Journal:Veterinary World -
The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
Journal:Journal of General and Family MedicineDOI:10.1002/jgf2.606 -
Nootropic effect of Syzygium polyanthum (Wight) Walp leaf extract in chronic cerebral hypoperfusion rat model via cholinergic restoration: a potential therapeutic agent for dementia
Journal:Advances in Traditional Medicine