AMPK alpha1/2 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat;Monkey
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB:K562 Verified Samples in IHC:Rat lung,Mouse testis |
| Dilution | WB 1:500-1:2000;IHC 1:100-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the non-phosphorylation site of Thr183/172. |
| Abbre | AMPKα1/2 |
| Synonyms | PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;抗体 |
| Swissprot | |
| Calculated MW | 62kDa |
| Observed MW | 63kDa |
| Cellular Localization | Cytosol,Nucleus,nuclear speck,nucleoplasm,nucleus,Plasma Membrane,apical plasma membrane,Other locations:cytoplasm,intracellular,nucleotide-activated protein kinase complex |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction |
| Conjugation | Unconjugated |
| Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |
Western Blot analysis of K562 cells using AMPK alpha1/2 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry of paraffin-embedded Rat lung tissue using AMPK alpha1/2 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse testis tissue using AMPK alpha1/2 Polyclonal Antibody at dilution of 1:200.
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
