Annexin V-FITC/PI Apoptosis Kit
中文名称:Annexin V-FITC / PI细胞凋亡检测试剂盒(即用型Buffer)
Price:
Size:
200Assays
100Assays
50Assays
20Assays
- 应用: 细胞凋亡-Annexin V
检测原理 | Annexin V是一种钙离子依赖性磷脂结合蛋白,与磷脂酰丝氨酸PS有高度亲和力,可通过细胞外侧暴露的PS与凋亡早期细胞胞膜结合,将Annexin V标记荧光染料,利用流式细胞仪检测细胞凋亡。
细胞核染料(PI,DAPI,7-AAD)可与双链DNA特异性结合,并产生强烈的荧光,正常情况下无法透过细胞膜。由于凋亡晚期或坏死细胞膜丧失完整性,细胞核染料(DAPI、PI、7-AAD)可进入细胞内对DNA进行染色,与Annexin V搭配使用,可区分处于不同凋亡时期的细胞。 |
检测方法 | 荧光;流式 |
样本类型 | 细胞 |
检测时间 | 40min |
检测仪器 | 流式细胞仪 |
荧光 | FITC |
Ex/Em | 490/530 |
Channel set | FITC |
自备试剂 | PBS(E-BC-R187), 去离子水 |
保存温度 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
运输条件 | 冰袋 |
-
Targeting the COX2/MET/TOPK signaling axis induces apoptosis in gefitinib-resistant NSCLC cells
IF: 5.959Journal:Cell Death & Disease -
Melatonin Downregulates PD-L1 Expression and Modulates Tumor Immunity in KRAS-Mutant Non-Small Cell Lung Cancer
IF: 5.924 -
Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia
IF: 5.875 -
Discovery of potent tubulin inhibitors targeting the colchicine binding site via structure-based lead optimization and antitumor evaluation
IF: 5.756Journal:JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY -
Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma
IF: 5.722 -
Cyanidin-3-O-Glucoside Ameliorates Palmitic-Acid-Induced Pancreatic Beta Cell Dysfunction by Modulating CHOP-Mediated Endoplasmic Reticulum Stress Pathways
IF: 5.719 -
Notoginsenoside R1 induces oxidative stress and modulates LPS induced immune microenvironment of nasopharyngeal carcinoma
IF: 5.714 -
LncRNA GATA3-AS1 promoted invasion and migration in human endometrial carcinoma by regulating the miR-361/ARRB2 axis
IF: 5.606 -
Long Noncoding RNA LINC01435 Impedes Diabetic Wound Healing by Facilitating YY1-Mediated HDAC8 Expression
IF: 5.458Journal:iScience -
Berberine suppresses colorectal cancer by regulation of Hedgehog signaling pathway activity and gut microbiota
IF: 5.34
E-CK-A111 | Annexin V-FITC Reagent | 500Tests , 200Tests , 100Tests , 50Tests |
E-CK-A151 | Annexin V Binding Buffer (10×) | 50mL , 10mL |
E-CK-A212 | Annexin V-FITC/7-AAD Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A252 | Annexin V-FITC/DAPI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |