ASC / TMS1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB:A549,THP-1,HT29,BT-474 Verified Samples in IF: HeLa |
| Dilution | WB 1:500-1:2000;IF 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein of human ASC / TMS1 (NP_037390.2). |
| Abbre | ASC / TMS1 |
| Synonyms | PYCARD;ASC;CARD5;TMS;TMS-1;TMS1;PYD and CARD domain containing;ASC / TMS1 |
| Swissprot | |
| Calculated MW | 15kDa/19kDa/21kDa |
| Observed MW | 22kDa |
| Cellular Localization | Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cell Biology |
| Conjugation | Unconjugated |
| Background | This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. |
Western blot analysis of extracts of various cell lines using ASC / TMS1 Polyclonal Antibody.
Immunofluorescence analysis of HeLa cells using ASC / TMS1 Polyclonal Antibody
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Formaldehyde aggravates allergic contact dermatitis by facilitating NLRP3 inflammasome activation in macrophages
IF: 5.714Journal：INTERNATIONAL IMMUNOPHARMACOLOGY -
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IF: 4.38 -
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