beta Tubulin Monoclonal Antibody
Price:
Size:
100μL
- 宿主： Mouse
- 反应性： Human; Mouse; E.coli; Scallop; Zebrafish
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB:Hela,Caco-2,HT-29,NIH/3T3,Raw264.7,Rat liver,HEK293 |
| Dilution | WB 1:5000-10000;IF 1:2000-4000 |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant human TUBB protein |
| Abbre | beta Tubulin |
| Synonyms | Beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; Beta1 tubulin; Class I beta tubulin; TUBB 1; TUBB 2; TUBB 5; TUBB; TUBB1; TUBB2; TUBB5; tubulin beta 1 chain; Tubulin beta 2 chain; tubulin beta 5 chain; Tubulin beta chain; Tubulin beta class I; tubulin beta polypeptide; Tubulin beta-5 chain抗体 |
| Swissprot | |
| Calculated MW | 55kDa |
| Observed MW | 55kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Microtubule |
| Concentration | 3mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide,50% glycerol,pH 7.4 |
| Purification Method | Protein A/G Purified |
| Clone No. | 3A7 |
| Conjugation | Unconjugated |
| Background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Western blotting with anti-Beta Tubulin monoclonal antibody at dilution of 1:5000.
Lane 1: Hela cell lysates, Lane 2: Caco-2 cell lysates, Lane 3: HT-29 cell lysates, Lane 4: NIH/3T3 cell lysates, Lane 5:Raw264.7 cell lysates, Lane 6: Rat liver
Immunofluorescent analysis of HEK293 cells with anti-beta Tubulin monoclonal antibody at dilution of 1:4000.
