Brdu Monoclonal Antibody
Price:
Size:
120μL
60μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat
- 应用性： IHC;IF
| Verified Samples |
Verified Samples in IHC:Mouse jejunum Verified Samples in IF: Hela |
| Dilution | IHC 1:100-1:500;IF 1:100-1:500 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated BrdU |
| Abbre | Brdu |
| Synonyms | Bromodeoxyuridine;Bromodeoxyuridine;5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.抗体 |
| Concentration | 0.7 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cell Biology;Neuroscience;Tags and Cell Markers;Epigenetics and Nuclear Signaling |
| Clone No. | 4B6C12 |
| Conjugation | Unconjugated |
| Background | Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. <br />
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Immunohistochemistry analysis of paraffin-embedded mouse jejunum(intraperitoneal injection BrdU every 2 hours for 4 times) using Brdu Monoclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of Hela cells（treated with 0.03mg/ml BrdU for 40 min ) using Brdu Monoclonal Antibody at dilution of 1:100.
