CASP9 Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human
- 应用性： WB;IHC-p;IF;IP
| Verified Samples |
Verified Samples in WB:Hela |
| Dilution | WB 1:500-1:2000;IHC 1:50-300;IF 1:50-1:200;IP 1:100-1:300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Abbre | Caspase 9 |
| Synonyms | APAF-3;APAF3;Apoptosis related cysteine peptidase;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP-9;CASP9;CASP9;Caspase 9 apoptosis related cysteine peptidase;Caspase 9 Dominant Negative;Caspase 9c;Caspase-9;Caspase-9 subunit p10;ICE LAP6;ICE like apoptotic protease 6;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1;regulatory subunit 56;RNCASP9抗体 |
| Swissprot | |
| Calculated MW | 46kDa |
| Observed MW | 47-51kDa |
| Cellular Localization | Cytosol,apoptosome,cytosol,Mitochondrion,Nucleus,Other locations:protein complex |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Cell Biology; Metabolism |
| Clone No. | Clone:3I3 |
| Conjugation | Unconjugated |
| Background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
Western Blot analysis of Hela cells using CASP9 Monoclonal Antibody at dilution of 1) 1:2000 2) 1:5000.
