EIF4A1 Monoclonal Antibody

说明书

Price: ¥2310 ¥1420 ¥580

Size:

120μL 60μL 20μL

Verified Samples	Verified Samples in WB:293T,Hela,HepG2,Mouse brain Verified Samples in IHC:Mouse colon Verified Samples in IF:Mouse spleen
Dilution	WB 1:500-1:3000;IHC 1:50-1:300;IF 1:100-1:200
Clonality	Monoclonal
Isotype	IgG
Immunogen	Synthetic Peptide
Abbre	eIF4A1
Synonyms	ATP-dependent RNA helicase eIF4A-1;DDX2;DDX2A;eIF 4A I;eIF-4A-I;EIF4A;eIF4A I;eIF4A-I;EIF4A1;Eukaryotic initiation factor 4A-I;Eukaryotic initiation factor 4AI;eukaryotic translation initiation factor 4A;isoform 1;Eukaryotic translation initiation factor 4A1;IF4A1抗体
Swissprot	P60842
Observed MW	48kDa
Cellular Localization	Cytosol,Extracellular region or secreted,extracellular exosome,extracellular matrix,Other locations:cytoplasm,eukaryotic translation initiation factor 4F complex,membrane
Concentration	1 mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method	Protein A purification
Research Areas	Cancer; Epigenetics and Nuclear Signaling
Clone No.	Clone:2B6
Conjugation	Unconjugated
Background	EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Western Blot analysis of 1) 293T, 2) Hela, 3) HepG2, 4) Mouse brain with eIF4A1 Monoclonal Antibody.

Immunohistochemistry of paraffin-embedded Mouse colon tissue using eIF4A1 Monoclonal Antibody at dilution of 1:200.

Immunofluorescence analysis of Mouse spleen tissue using eIF4A1 Monoclonal Antibody at dilution of 1:200.

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500μL , 120μL , 1mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	18mL , 6mL , 3mL
E-IR-R220	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50Assays