GAPDH Polyclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat;Zebrafish
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB:Zebrafish,Hela,A431,A549,Rat brain,Rat kidney,Mouse brain,Mouse kidney Verified Samples in IF: 293 |
| Dilution | WB 1:1000-2000;IF 1:100-400 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Abbre | GAPDH |
| Synonyms | aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component抗体 |
| Swissprot | |
| Calculated MW | 40.2kDa |
| Observed MW | 36kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 2mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer; Metabolism; Signal Transduction |
| Conjugation | Unconjugated |
| Background | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
Western blot of Zebrafish whole lysates with anti-GAPDH at dilution of 1:2000.
Immunofluorescence analysis of 293 cell, using anti-GAPDH Polyclonal Antibody at 1:400 dilution.
