GFP Monoclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主: Mouse
- 反应性: All
- 应用性: WB;IF;IP;FCM
Verified Samples |
Verified Samples in WB:eGFP,eGFP protein with anti-eGFP Verified Samples in IF:zebrafish embryo Verified Samples in IP:293 Verified Samples in FCM:CHO |
Dilution | WB 1:100000-200000;IP 3ug/sample;IF 1:8000-16000;FCM 0.2ug |
Clonality | Monoclonal |
Isotype | IgG2a |
Immunogen | Recombinant GFP protein expressed by E.coli |
Abbre | GFP-Tag |
Synonyms | GFP;Green fluorescent protein抗体 |
Swissprot | |
Calculated MW | 32kDa |
Observed MW | 32kDa |
Concentration | 1mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
Purification Method | Protein A/G Purified |
Clone No. | 5B5 |
Conjugation | Unconjugated |
Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Western blot of eGFP protein with anti-eGFP at dilution of 1:80000.
Immunohistochemical analysis of transgenic zebrafish embryo with anti-GFP antibody at dilution of 1:800.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:8000.
IP Result of 293 cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
1x106 CHO cells Transfected with a GFP plasmid were stained with 0.2ug Anti-GFP tag mAb(E-AB-48010) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
-
Promoter Binding and Nuclear Retention Features of Zebrafish IRF Family Members in IFN Response
IF: 7.3 -
Translational control by maternal Nanog promotes oogenesis and early embryonic development
IF: 6.21 -
High-efficiency transformation of a centric diatom Chaetoceros muelleri by electroporation with a variety of selectable markers
IF: 5Journal:Algal Research -
O-GlcNAc transferase promotes the nuclear localization of the focal adhesion-associated protein Zyxin to regulate UV-induced cell death
IF: 4.8 -
Molecular and functional identification of a short-type peptidoglycan recognition protein, PGRP-S, in the Chinese soft-shelled turtle Pelodiscus sinensis
IF: 2.9