Glutathione Peroxidase (GSH-Px) Activity Assay Kit
中文名称:谷胱甘肽过氧化物酶(GSH-Px)比色法测试盒
Price:
Size:
96T(40 samples)
48T(16 samples)
- 样本类型: 血清(浆);动植物组织;细胞;培养液
- 检测仪器及波长: 酶标仪(400-420 nm,最佳检测波长412 nm)
- 研究领域: 氧化应激,植物生理系列,铁死亡
该指标在测定组织或细胞样本时,需要测定样本的总蛋白含量,推荐使用BCA法测蛋白,货号E-BC-K318-M
说明书或网站上未涉及的样本类型请先跟技术支持沟通,可进行售前验证
根据需测样本数,试剂盒测样本孔数和单个样本测量孔数来计算所需生化试剂盒数量,请点击按钮 计算
生化试剂盒数量计算器x
需测样本数:
单个样本测量孔数:
试剂盒测样本孔数:
- 16
- 18
- 19
- 22
- 23
- 24
- 25
- 32
- 36
- 40
- 42
- 43
- 44
- 46
- 48
- 50
- 80
- 86
- 92
- 96
- 100
- 480
x
所需盒子量:
检测原理 |
GSH-Px可以促进过氧化氢(H2O2)与还原型谷胱甘肽(GSH)反应生成H2O及氧化型谷胱甘肽(GSSG),反应式见下图。通过测定底物GSH在单位时间内被氧化的速度来表示酶的活力,反应剩余的GSH与二硫代双二硝基苯甲酸(DTNB)作用生成的黄色硫代硝基苯甲酸阴离子(TNB),测定该阴离子的浓度来计算出GSH的减少量。由于GSH在没有酶的条件下也能进行氧化(非酶反应),所以最后计算此酶活力时,必须扣除非酶反应引起的GSH减少的那部分。
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指标简称 | GSH-Px |
灵敏度 | 17.17 U |
检测范围 | 17.17-518.32 U |
精密度 | 批间差:6.2% | 批内差:2.4% |
检测时间 | 40 min |
检测仪器及波长 | 酶标仪(400-420 nm,最佳检测波长412 nm) |
保存条件 | 该产品可在 2~8°C避光条件下保存6个月。 |
有效期 | 6个月 |
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