HIS(Histamine) ELISA Kit
中文名称:组胺(HIS)酶联免疫吸附测定试剂盒
别称:
HIS
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用HIS抗原包被于酶标板上,实验时样品或标准品中的HIS与包被的HIS竞争生物素标记的抗HIS单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,HIS浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中HIS的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的HIS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
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ORMDL3 Functions as a Negative Regulator of Antigen-Mediated Mast Cell Activation via an ATF6-UPR-Autophagy–Dependent Pathway
IF: 7.561Journal:Frontiers in Immunology -
A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols
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Propofol pretreatment alleviates mast cell degranulation by inhibiting SOC to protect the myocardium from ischemia–reperfusion injury
IF: 6.53Journal:BIOMEDICINE & PHARMACOTHERAPY -
Hypoallergenic derivatives of Scylla paramamosain heat-stable allergens alleviated food allergy symptoms in Balb/c mice
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Fullerene nanoparticles for the treatment of ulcerative colitis
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Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
IF: 5.924 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719 -
Establishment of Food Allergy Model in Dextran Sulfate Sodium Induced Colitis Mice
IF: 5.561Journal:Foods -
Strong Histamine Torsion Raman Spectrum Enables Direct, Rapid and Ultrasensitive Detection of Allergic Diseases
IF: 5.458Journal:iScience