HIS(Histamine) ELISA Kit
中文名称:组胺(HIS)酶联免疫吸附测定试剂盒
别称:
HIS
Price:
Size:
96T
48T
96T*5
- 反应性: Universal
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用竞争ELISA法。用HIS抗原包被于酶标板上,实验时样品或标准品中的HIS与包被的HIS竞争生物素标记的抗HIS单抗上的结合位点,游离的成分被洗去。加入辣根过氧化物酶标记的亲和素,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,HIS浓度与OD450值之间呈反比,通过绘制标准曲线计算出样品中HIS的浓度。 |
反应类型 | Competitive-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 2.5h |
反应性 | Universal |
检测方法 | Colormetric |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 50μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的HIS,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice
IF: 5.279 -
Reducing the Allergenicity of α-Lactalbumin after Lipid Peroxidation
IF: 5.279 -
Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice
IF: 4.781Journal:PLoS Neglected Tropical Diseases -
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Mechanistic Studies of the Antiallergic Activity of Phyllanthus amarus Schum. & Thonn. and Its Compounds
IF: 4.412Journal:MOLECULES -
Effects of Deacetylasperulosidic Acid on Atopic Dermatitis through Modulating Immune Balance and Skin Barrier Function in HaCaT, HMC-1, and EOL-1 Cells
IF: 4.412 -
Biochemical analysis reveals the systematic response of motion sickness mice to ginger (Zingiber officinale) extract's amelioration effect
IF: 4.36 -
Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge
IF: 4.047Journal:Parasites & Vectors -
Screening of probiotic Lactobacilli with potential anti-allergic activity based on hyaluronidase inhibition and degranulation of RBL-2H3 cells in vitro
IF: 4.006Journal:LWT-FOOD SCIENCE AND TECHNOLOGY -
Protective Effects of Live Combined B. subtilis and E. faecium in Polymicrobial Sepsis Through Modulating Activation and Transformation of Macrophages and Mast Cells
IF: 3.845