His-Tag Monoclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB;IP;FCM
| Verified Samples |
Verified Samples in WB:His-Tag fused recombinant protein Verified Samples in IP:293F Verified Samples in FCM: CHO |
| Dilution | WB 1:1000-2000;IP 3ug/sample;FCM 0.2ug |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag; Polyhistidine Tag抗体 |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.1% sodium azide, 50% glycerol, pH 7.4 |
| Purification Method | Protein A/G Purified |
| Clone No. | 1A5 |
| Conjugation | Unconjugated |
| Background | The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world. |
Western blot of His-Tag fused recombinant protein with Anti-His mouse monoclonal antibody at dilution of 1:1000.
IP Result of 293F cells transfected with His-Tag fusion protein,using anti-His-Tag mouse monoclonal antibody . Lane 1:input, Lane 2: anti-His-Tag mouse monoclonal antibody, Lane 3: mouse IgG1 Isotype Control
1x106 CHO cells Transfected with a His plasmid were stained with 0.2ug Anti-His tag mAb(E-AB-48003) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1011 | Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1015 | Goat Anti-Mouse IgG(H+L)(FITC conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1059 | Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1076 | Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1087 | Elab Fluor® 405-Goat Anti-Mouse IgG(H+L) | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
