His-Tag Polyclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Rabbit
- 反应性： All
- 应用性： WB
| Verified Samples |
Verified Samples in WB:6xHis-Tagged fused protein,HEK 293 |
| Dilution | WB 1:1000-2000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin. |
| Abbre | His-Tag |
| Synonyms | 6His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag;His tag; Polyhistidine Tag抗体 |
| Calculated MW | 28kDa |
| Observed MW | 28kDa |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | The His-tag (also called 6xHis-tag) is one of the simplest and most widely used purification tags, with six or more consecutive histidine residues. It is the most widely used protein affinity tag. Due to its small size of only six amino acids it rarely in |
Western blot with anti-6xHis antibody at dilution of 1:2000.
lane 1: HEK 293 whole cell lysate, lane 2: 6xHis tag transfected HEK 293 cell lysates
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
