Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
中文名称:人脑源性神经营养因子(BDNF)酶联免疫吸附测定试剂盒
别称:
ANON2、BULN2
Price:
Size:
96T
48T
96T*5
- 反应性: Human
- 检测范围: 31.25-2000 pg/mL
- 灵敏度: 18.75 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人BDNF抗体包被于酶标板上,实验时样品(或标准品)中的人BDNF会与包被抗体结合。后依次加入生物素化的抗人BDNF抗体和辣根过氧化物酶标记的亲和素,抗人BDNF抗体与结合在包被抗体上的人BDNF结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,BDNF浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中BDNF的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Human |
检测方法 | Colormetric |
检测范围 | 31.25-2000 pg/mL |
灵敏度 | 18.75 pg/mL |
样本体积 | 100μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的人BDNF,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
Effect of levetiracetam in combination with topiramate on immune function, cognitive function, and neuronal nutritional status of children with intractable epilepsy
IF: 4.06Journal:American Journal of Translational ResearchPMID:34650715 -
Schizophrenia-associated rs4702 G allele-specific downregulation of FURIN expression by miR-338-3p reduces BDNF production
IF: 3.958Journal:SCHIZOPHRENIA RESEARCH -
Interleukin-6, undercarboxylated osteocalcin, and brain-derived neurotrophic factor responses to single and repeated sessions of high-intensity interval exercise
IF: 3.867 -
Association of blood lead levels with neurobehavior and BDNF expression in school going children
IF: 3.849Journal:JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGYPMID:33773278 -
Altered levels of neurobiological biomarkers at the interface of depression and gestational diabetes mellitus in Asian Indian women
IF: 3.286 -
Longitudinal Investigation of Psychotherapy Outcomes (LIPO): Description of the Study Protocol
IF: 3.161Journal:Frontiers in Psychiatry -
Neuroprotective effects of brain‐derived neurotrophic factor against amyloid beta 1‐40‐induced retinal and optic nerve damage
IF: 3.115Journal:EUROPEAN JOURNAL OF NEUROSCIENCE -
Left lateral parietal rTMS improves cognition and modulates resting brain connectivity in patients with Alzheimer’s disease: Possible role of BDNF and oxidative stress
IF: 2.877Journal:NEUROBIOLOGY OF LEARNING AND MEMORY -
The effects of six months Persicaria minor extract supplement among older adults with mild cognitive impairment: a double-blinded, randomized, and placebo-controlled trial
IF: 2.833 -
Association of BDNF levels with IQ: comparison of S100B and BDNF levels in typically developing children and subjects with neurologically normal nonsyndromic intellectual disability
IF: 2.424