MCM3 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:HepG2,PC-12,C6,Raw264.7,NIH/3T3 Verified Samples in IHC:Human lymph node,Human tonsil |
| Dilution | WB 1:500-1:1000;IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Human DNA replication licensing factor MCM3 protein |
| Abbre | MCM3 |
| Synonyms | Cervical cancer proto oncogene 5;DNA polymerase alpha holoenzyme associated P1;HCC 5;HCC5;hRlf beta subunit;Human cervical cancer proto oncogene 5;MCM 3;MGC1157;Minichromosome maintenance complex component 3;P1 h;P1 MCM3;P1.h;p102;Replication licensing factor beta subunit;
RLF beta subunit;RLFB抗体 |
| Swissprot | |
| Calculated MW | 91,96 kDa |
| Observed MW | 100 kDa |
| Cellular Localization | Chromosome, Nucleus |
| Concentration | 2mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Background | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with mCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. |
Western Blot analysis of HepG2 cell using MCM3 Polyclonal Antibody at dilution of 1:500
Western blotting with anti-MCM3 polyclonal antibody at dilution of 1:1000. Lane 1:PC-12 cell lysate,Lane 2:C6 cell lysate,lane 3:Raw264.7 cell lysate,lane 4:NIH/3T3 cell lysate.
Immunohistochemistry of paraffin-embedded Human lymph node using MCM3 Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human tonsil using MCM3 Polyclonal Antibody at dilution of 1:200(Elabscience Product Detected by Lifespan).
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
