MRPL42 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Jurkat,HepG2,Hela,Raji Verified Samples in IHC:Human cervical cancer,Human colorectal cancer |
| Dilution | WB 1:1000-1:5000;IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human MRPL42 |
| Abbre | MRPL42 |
| Synonyms | 28S ribosomal protein S32;39S ribosomal protein L31;39S ribosomal protein L42;HSPC204 ;L31mt;L42mt;mitochondrial;Mitochondrial ribosomal protein L42;Mitochondrial ribosomal protein S32;MRP L31;MRP L42;MRP S32 ;MRP-L31;MRP-L42;MRP-S32;MRPL31 ;Mrpl42;MRPS32 ;PTD007 ;RM42;RPML31 ;S32mt |
| Swissprot | |
| Calculated MW | 17 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Mitochondrion. |
| Concentration | 1.14 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling; Metabolism |
| Conjugation | Unconjugated |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. |
Western blot analysis of Jurkat HepG2 Hela and Raji cell lysatesusing MRPL42 Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human cervical cancer tissueusing MRPL42 Polyclonal Antibody at dilution of 1:65(×200)
Immunohistochemistry of paraffin-embedded Human colorectal cancer tissueusing MRPL42 Polyclonal Antibody at dilution of 1:65(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
