MS Mouse IL-12(Interleukin 12) ELISA Kit
中文名称:MS-小鼠白介素12(IL-12)酶联免疫吸附测定试剂盒
别称:
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 0.31-20 pg/mL
- 灵敏度: 0.19 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-12浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-12抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-12会与包被抗体结合。后依次加入生物素化的抗小鼠IL-12抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-12抗体与结合在包被抗体上的小鼠IL-12结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-12浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-12的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.31-20 pg/mL |
灵敏度 | 0.19 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-12,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
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Recombinant Listeria ivanovii strain expressing listeriolysin O in place of ivanolysin O might be a potential antigen carrier for vaccine construction
IF: 6.064 -
Deacetylasperulosidic Acid Ameliorates Pruritus, Immune Imbalance, and Skin Barrier Dysfunction in 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis NC/Nga Mice
IF: 5.924 -
Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice
IF: 5.719 -
Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency
IF: 5.545 -
λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine
IF: 5.168Journal:Oncotarget -
Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis
IF: 5.008Journal:Oncotarget -
Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis
IF: 4.868 -
Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy
IF: 4.845Journal:INTERNATIONAL JOURNAL OF PHARMACEUTICS -
Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function
IF: 4.546 -
Defibrotide inhibits donor leucocyte‐endothelial interactions and protects against acute graft‐versus‐host disease
IF: 4.486Journal:JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
E-EL-M3062 | Mouse IL-12(Interleukin 12) ELISA Kit | 96T , 48T , 96T*5 |