MS Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称:MS-小鼠白介素1β(IL-1β)酶联免疫吸附测定试剂盒
别称:
IL1B、IL1-BETA、IL1F2、Catabolin
Price:
Size:
96T
48T
96T*5
- 反应性: Mouse
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 3.55 pg/mL
系列介绍 | MS为Mini Sample的缩写。MS系列试剂盒的上样量仅为传统夹心法ELISA试剂盒的1/4,能有效解决实验样本体积不够的问题。Elabscience®自主开发的新技术,旨在帮助您以更高效的方式进行科学研究。 |
用途 | 试剂盒用于体外定量检测小鼠血清、血浆或其他生物体液中IL-1β浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-1β抗体包被于酶标板上,实验时样品(或标准品)中的小鼠IL-1β会与包被抗体结合。后依次加入生物素化的抗小鼠IL-1β抗体和辣根过氧化物酶标记的亲和素,抗小鼠IL-1β抗体与结合在包被抗体上的小鼠IL-1β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96T
/ 48T
/ 96T*5
|
反应时间 | 3.5h |
反应性 | Mouse |
检测方法 | Colormetric |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 3.55 pg/mL |
样本体积 | 25μL |
样本类型 | 血清,血浆,细胞上清,细胞提取液体,组织匀浆等其他生物体液 |
特异性 | 可检测样本中的小鼠IL-1β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃/-20℃ |
数据处理 |
-
MicroRNA-377-3p released by mesenchymal stem cell exosomes ameliorates lipopolysaccharide-induced acute lung injury by targeting RPTOR to induce autophagy
IF: 8.469Journal:Cell Death & Disease -
Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32
IF: 8.469 -
Sirtuin 3 protects against anesthesia/surgery-induced cognitive decline in aged mice by suppressing hippocampal neuroinflammation
IF: 8.322 -
Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer’s disease-like mice
IF: 8.322Journal:Journal of Neuroinflammation -
Structural characterization of a soluble polysaccharide SSPS1 from soy whey and its immunoregulatory activity in macrophages
IF: 8.025Journal:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULESPMID:35835298 -
Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe
IF: 8.008 -
Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway
IF: 7.963 -
Comprehensive Effect of Carbon Tetrachloride and Reversal of Gandankang Formula in Mice Liver: Involved in Oxidative Stress, Excessive Inflammation, and Intestinal Microflora
IF: 7.675Journal:Antioxidants -
Oxidized chondroitin sulfate eye drops ameliorate the prognosis of fungal keratitis with anti-inflammatory and antifungal effects
IF: 7.571 -
PHLDA1 Suppresses TLR4-Triggered Proinflammatory Cytokine Production by Interaction With Tollip
IF: 7.561Journal:Frontiers in Immunology
E-EL-M0037 | Mouse IL-1β(Interleukin 1 Beta) ELISA Kit | 96T , 48T , 96T*5 |