p53RFP Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:TM4 Verified Samples in IHC:Human lung cancer,Human cervical cancer |
Dilution | WB 1:200-1:1000;IHC 1:50-1:200 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant protein of human RNF144B |
Abbre | RNF144B |
Synonyms | bA528A10.3;E3 ubiquitin protein ligase RNF144B;E3 ubiquitin-protein ligase RNF144B;IBR domain containing 2;IBR domain containing protein 2;IBR domain-containing protein 2;IBRDC 2;IBRDC2;KIAA0161;MGC71786;OTTHUMP00000016079;OTTHUMP00000016080;OTTHUMP00000039360;p53 inducible RING finger protein;p53-inducible RING finger protein;p53RFP;PIR2;R144B;Ring finger 144B;RING finger protein 144B;RNF 144B;RNF144B抗体 |
Swissprot | |
Calculated MW | 34kDa |
Cellular Localization | Mitochondrion membrane. Cytoplasm. Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation. |
Concentration | 0.4 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
Purification Method | Affinity purification |
Research Areas | Cell Biology |
Conjugation | Unconjugated |
Background | p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle. |
Western Blot analysis of TM4 cell using p53RFP Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human lung cancer using p53RFP Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human cervical cancer using p53RFP Polyclonal Antibody at dilution of 1:50
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