PCNA Monoclonal Antibody

说明书

Price: ¥1280 ¥800 ¥499 ¥219

Size:

200μL 100μL 50μL 20μL

Verified Samples	Verified Samples in WB:Zebrafish Verified Samples in IHC: Zebrafish
Dilution	WB 1:1000-2000
Clonality	Monoclonal
Isotype	IgG1
Immunogen	Recombinant human PCNA protein expressed by E.coli
Abbre	PCNA
Synonyms	ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; Pcna/cyclin; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03抗体
Swissprot	P12004
Calculated MW	30kDa
Observed MW	36kDa
Cellular Localization	Nucleus
Concentration	2mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Purification Method	Protein A/G Purified
Research Areas	Cancer; Cell Biology; Epigenetics and Nuclear Signaling; Tags and Cell Markers
Clone No.	8B12
Conjugation	Unconjugated
Background	Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test.

Western blot of Zebrafish whole lysates with anti-PCNA monoclonal antibody at dilution of 1:1000.

Immunofluorescence of Zebrafish frozen section with anti-PCNA monoclonal antibodyat dilution of 1:100.

Immunohistochemistry of Zebrafish paraffin embedded section with anti-PCNA mcab at dilution of 1:100.

E-AB-1001	Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1011	Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1015	Goat Anti-Mouse IgG(H+L)(FITC conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1024	Goat Anti-Mouse IgG(H+L)(Biotin conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1059	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1076	Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated)	500μL , 120μL , 60μL , 30μL , 1mL
E-AB-1196	HRP-Goat Anti-Mouse IgG(H+L) preadsorbed	500μL , 120μL , 1mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	18mL , 6mL , 3mL
E-IR-R220	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50Assays