PI 3 kinase p85 alpha Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Mouse;Rat
- 应用性： WB;IHC-p
| Verified Samples |
Verified Samples in WB:3T3,Rat liver Verified Samples in IHC:Rat liver,Mouse brain |
| Dilution | WB 1:1000-2000;IHC 1:100-200 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein |
| Abbre | PI3 Kinase P85α |
| Synonyms | GRB1;p85 alpha;p85;P85A;Phosphatidylinositol 3 kinase associated p 85 alpha;Phosphatidylinositol 3 kinase regulatory 1;Phosphatidylinositol 3 kinase;regulatory subunit;polypeptide 1 (p85 alpha);Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;Phosphatidylinositol 3-kinase regulatory subunit alpha;Phosphoinositide 3 kinase;regulatory subunit 1 (alpha);PI3 kinase p85 subunit alpha;PI3-kinase regulatory subunit alpha;PI3-kinase subunit p85-alpha;PI3K;PI3K regulatory subunit alpha;Pik3r1;PtdIns 3 kinase p85 alpha;PtdIns-3-kinase regulatory subunit alpha;PtdIns-3-kinase regulatory subunit p85-alpha抗体 |
| Swissprot | |
| Observed MW | 85kDa |
| Cellular Localization | Cytosol,Endoplasmic reticulum,perinuclear endoplasmic reticulum membrane,Nucleus,Plasma Membrane,Other locations:cell-cell junction,cis-Golgi network,cytoplasm,membrane,phosphatidylinositol 3-kinase complex,phosphatidylinositol 3-kinase complex, class IA,protein complex |
| Tissue Specificity | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Metabolism; Immunology; Signal Transduction |
| Clone No. | 3B7 |
| Conjugation | Unconjugated |
| Background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. |
Western Blot analysis of 1) 3T3, 2) Rat liver using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Rat liver tissue using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Mouse brain tissue using PI 3 kinase p85 alpha Monoclonal Antibody at dilution of 1:200.
| E-AB-1001 | Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1024 | Goat Anti-Mouse IgG(H+L)(Biotin conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1196 | HRP-Goat Anti-Mouse IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
