Recombinant E.coli Beta-Glucuronidase/Uida/Beta-Gus Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P05804
| 别称 |
Beta-glucuronidase;uidA;GUS
|
| 表达系统 |
E.coli
|
| 序列 |
Met1-Gln603
|
| 蛋白编码 |
P05804
|
| 种属 |
E.coli
|
| 计算分子量 |
69.9 kDa
|
| 表观分子量 |
72 kDa
|
| 标签 |
N-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Beta-D-glucuronidase from E.coli is a ?highly specific enzyme in hydrolyzing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiazepines, steroids, cannabinoids, and others.
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实验操作视频
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