Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)
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- 表达系统： E.coli
- 蛋白编码： P03023
| 别称 |
Lactose operon repressor;LacI
|
| 表达系统 |
E.coli
|
| 序列 |
Met1-Gln360
|
| 蛋白编码 |
P03023
|
| 种属 |
E.coli
|
| 计算分子量 |
39.4 kDa
|
| 表观分子量 |
40 kDa
|
| 标签 |
C-His
|
| 纯度 |
> 95 % as determined by reducing SDS-PAGE.
|
| 内毒素 |
< 1.0 EU per μg of the protein as determined by the LAL method.
|
| 保存条件 |
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
|
| 运输条件 |
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
|
| 制剂 |
Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
|
| 复溶方法 |
Not Applicable
|
| 背景 |
Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
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实验操作视频
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